Abdulah Rizky, Milanda Tiana, Sugijanto Milyadi, Barliana Melisa I, Diantini Ajeng, Supratman Unang, Subarnas Anas
Southeast Asian J Trop Med Public Health. 2017 Jan;48(1):109-16.
Bacterial antimicrobial resistance is a major health problem worldwide. Plants consumed by non-human primates are potentially safe for humans. In this study, we examined the potential antibacterial properties of plants consumed by non-human primates in Indonesia. We studied the antibacterial properties of the leaf extracts of 34 primate-consumed plants against Escherichia coli and Bacillus subtilis in vitro. The plants were collected from the Pangandaran Conservation Area, West Java Province, Indonesia. The leaves were dried and then powdered by crushing and the potential active ingredients were extracted with 95% ethanol at room temperature for 24 hours. The obtained solvent was then dried at 50ºC under reduced pressure. The antibacterial properties of each product were then tested to determine the minimum inhibitory and minimum bactericidal concentrations using the broth microdilution technique and a disc diffusion test was also performed. The results show Kleinhovia hospita, Dillenia excelsa and Garcinia celebica had the best antibacterial properties against Escherichia coli and Ficus benjamina, Ficus altissima, and Elaeocarpus glaber had the best antibacterial properties against Bacillus subtilis. Some of the studied leaf extracts in our study have the potential to be developed into antibacterial medications and need to be studied further.
细菌抗药性是全球主要的健康问题。非人灵长类动物食用的植物对人类可能是安全的。在本研究中,我们检测了印度尼西亚非人灵长类动物食用的植物的潜在抗菌特性。我们在体外研究了34种灵长类动物食用植物的叶提取物对大肠杆菌和枯草芽孢杆菌的抗菌特性。这些植物采自印度尼西亚西爪哇省的庞岸达兰保护区。叶子经干燥后通过研磨制成粉末,并在室温下用95%乙醇提取潜在的活性成分24小时。然后将所得溶剂在50ºC减压干燥。然后使用肉汤微量稀释技术测试每种产品的抗菌特性,以确定最低抑菌浓度和最低杀菌浓度,并且还进行了纸片扩散试验。结果表明,绒毛番龙眼、五桠果和西加里曼丹藤黄对大肠杆菌具有最佳抗菌特性,而垂叶榕、高山榕和秃瓣杜英对枯草芽孢杆菌具有最佳抗菌特性。我们研究中的一些叶提取物有潜力被开发成抗菌药物,需要进一步研究。