Napiroon Tiwtawat, Balslev Henrik, Duangjai Sutee, Sookchaloem Duangchai, Chayamarit Kongkanda, Santimaleeworagun Wichai, Vajrodaya Srunya
Southeast Asian J Trop Med Public Health. 2017 Jan;48(1):117-23.
Two tropical plant species, Lasianthus pilosus and Lasianthus stipularis are used in traditional medicine but there have been no published studies of the extracts of these plants against bacteria. In this study, we aimed to determine the antimicrobial activities of the above two plants against two gram-positive and seven gram-negative bacterial strains to determine the potential of these two plant species for possible antimicrobial drug development. The antibacterial activities of the lipophilic extracts of these plants were evaluated by disk diffusion and broth microdilution methods. The zone diameters and minimum inhibitory concentrations (MIC) for these plant extracts exhibited their highest antibacterial activity against Pseudomonas aeruginosa, followed by Staphylococcus aureus and Acinetobacter baumannii, respectively. The MIC of these extracts against P. aeruginosa (ATCC 37166 and ATCC 27853) varied from 50 to 200 μg/ml. Thin layer chromatography and detection using different specific reagents revealed the presence of terpenoids, phenolic compounds and iridoid. Cell lysis due to the effect of the lipophilic extracts of these tested plants was demonstrated using scanning electron microscopy. In conclusion, the bioactive compounds of these plants should be studied further to develop potential antimicrobial agents.
两种热带植物，毛萼九节和具托叶九节，被用于传统医学，但尚未有关于这些植物提取物抗菌作用的公开研究。在本研究中，我们旨在测定上述两种植物对两种革兰氏阳性菌和七种革兰氏阴性菌菌株的抗菌活性，以确定这两种植物在开发潜在抗菌药物方面的潜力。通过纸片扩散法和肉汤微量稀释法评估了这些植物亲脂性提取物的抗菌活性。这些植物提取物的抑菌圈直径和最低抑菌浓度（MIC）对铜绿假单胞菌表现出最高的抗菌活性，其次分别是金黄色葡萄球菌和鲍曼不动杆菌。这些提取物对铜绿假单胞菌（ATCC 37166和ATCC 27853）的MIC在50至200μg/ml之间。薄层色谱法和使用不同特定试剂的检测显示存在萜类化合物、酚类化合物和环烯醚萜。使用扫描电子显微镜证明了这些受试植物的亲脂性提取物具有细胞裂解作用。总之，应进一步研究这些植物的生物活性化合物，以开发潜在的抗菌剂。
