Institute of Fluid Dynamics , Helmholtz-Zentrum Dresden-Rossendorf (HZDR) , 01328 Dresden , Germany.
Medizinische Klinik und Poliklinik I , University Hospital Carl Gustav Carus der Technischen Universität Dresden , Fetscherstraße 74 , 01307 Dresden , Germany.
ACS Appl Mater Interfaces. 2018 May 2;10(17):14418-14425. doi: 10.1021/acsami.8b01029. Epub 2018 Apr 23.
Cross-linking biomolecules with electroconductive nanostructures through noncovalent interactions can result in modular networks with defined biological functions and physical properties such as electric conductivity and viscoelasticity. Moreover, the resulting matrices can exhibit interesting features caused by the dynamic assembly process, such as self-healing and molecular ordering. In this paper, we present a physical hydrogel system formed by mixing peptide-polyethylene glycol and poly(3,4-ethylenedioxythiophene):polystyrene sulfonate. This combinatorial approach, which uses different modular building blocks, could lead to high tunability on aspects of rheology and electrical impedance. The proposed physical hydrogel system is characterized by both a self-healing ability and injectability. Interestingly, the formation of hydrogels at relatively low concentrations led to a network of closer molecular packing of poly(3,4-ethylenedioxythiophene) nanoparticles, reflected by the enhanced conductivity. The biopolymer system can be used to develop three-dimensional cell cultures with incorporated electric stimuli, as evidenced by its contribution to the survival and proliferation of encapsulated mesenchymal stromal cells and their differentiation upon electrical stimulation.
通过非共价相互作用将生物分子与导电纳米结构交联，可以得到具有特定生物学功能和物理性质（如导电性和粘弹性）的模块化网络。此外，所得的基质可以表现出由动态组装过程引起的有趣特征，例如自修复和分子有序化。在本文中，我们提出了一种由混合肽-聚乙二醇和聚（3,4-亚乙基二氧噻吩）：聚苯乙烯磺酸盐形成的物理水凝胶系统。这种使用不同模块构建块的组合方法，可以在流变学和电阻抗方面实现高度的可调性。所提出的物理水凝胶系统具有自修复能力和可注射性。有趣的是，在相对较低的浓度下形成水凝胶导致聚（3,4-亚乙基二氧噻吩）纳米颗粒的分子排列更加紧密的网络，这反映在导电性的增强上。该生物聚合物系统可用于开发具有内置电刺激的三维细胞培养物，这一点可以通过其对封装间充质基质细胞的存活和增殖及其在电刺激下的分化的贡献来证明。
