Iuchi S, Lin E C
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA 02115.
Proc Natl Acad Sci U S A. 1988 Mar;85(6):1888-92. doi: 10.1073/pnas.85.6.1888.
In Escherichia coli the levels of numerous enzymes associated with aerobic metabolism are decreased during anaerobic growth. In an arcA mutant the anaerobic levels of these enzymes are increased. The enzymes, which are encoded by different regulons, include members that belong to the tricarboxylic acid cycle, the glyoxylate shunt, the pathway for fatty acid degradation, several dehydrogenases of the flavoprotein class, and the cytochrome o oxidase complex. Transductional crosses placed the arcA gene near min O on the chromosomal map. Complementation tests showed that the arcA gene corresponded to the dye gene, which is also known as fexA, msp, seg, or sfrA because of various phenotypic properties [Bachmann, B. (1983) Microbiol. Rev. 47, 180-230]. A dye-deletion mutant was derepressed in the aerobic enzyme system. The term modulon is proposed to describe a set of regulons that are subject to a common transcriptional control.
在大肠杆菌中，与有氧代谢相关的多种酶的水平在厌氧生长期间会降低。在arcA突变体中，这些酶的厌氧水平会升高。这些由不同调节子编码的酶包括属于三羧酸循环、乙醛酸循环支路、脂肪酸降解途径的成员、几种黄素蛋白类脱氢酶以及细胞色素o氧化酶复合体。转导杂交将arcA基因定位在染色体图谱上靠近min O的位置。互补试验表明，arcA基因与dye基因相对应，由于各种表型特性，dye基因也被称为fexA、msp、seg或sfrA [巴赫曼，B.（1983年）《微生物学评论》47，180 - 230]。一个dye缺失突变体在有氧酶系统中去阻遏。提出“调节子元”这一术语来描述一组受共同转录控制的调节子。
