Dietz J R
Department of Physiology and Biophysics, University of South Florida, College of Medicine, Tampa 33612.
Proc Soc Exp Biol Med. 1988 Mar;187(3):366-9. doi: 10.3181/00379727-187-42678.
Studies in intact animals have suggested that angiotensin II (AII) and antidiuretic hormone (ADH) increase the plasma concentration of atrial natriuretic factor (ANF). The purpose of these studies was to examine the effects of AII and ADH on ANF secretion in a rat heart-lung preparation under conditions where aortic pressure could be regulated and other indirect effects of these hormones eliminated. ANF secretion was estimated as the total amount of ANF present in a perfusion reservoir at the end of each 30-min period. A pump was used to deliver a fluorocarbon perfusate to the right atrium at rates of either 2 or 5 ml/min. In a time control series where venous return was maintained at 2 ml/min for three 30-min periods ANF secretion was 672 +/- 114, 794 +/- 91, and 793 +/- 125 pg/min (n = 6, P greater than 0.05). When venous return was increased from 2 to 5 ml/min ANF secretion increased from 669 +/- 81 to 1089 +/- 127 pg/min (P less than 0.01). The addition of AII to the perfusate in concentrations of 50, 100, or 200 pg/ml (n = 6 in each group) had no significant effect on basal ANF secretion or the ANF response to increasing venous return. Similarly, the addition of ADH to the perfusate in concentrations of 5, 25, or 100 pg/ml had no significant effect on ANF release from the heart. These results suggest that the ability of AII and ADH to increase plasma ANF concentration in vivo may be due to the effects of these hormones on right or left atrial pressure.
对完整动物的研究表明，血管紧张素II（AII）和抗利尿激素（ADH）可提高心房利钠因子（ANF）的血浆浓度。这些研究的目的是在可调节主动脉压力并消除这些激素其他间接影响的条件下，研究AII和ADH对大鼠心肺制备物中ANF分泌的影响。ANF分泌量通过每个30分钟时段末灌注储液器中存在的ANF总量来估算。使用泵以2或5 ml/min的速率将氟碳灌注液输送至右心房。在一个时间对照系列中，静脉回流量在三个30分钟时段内维持在2 ml/min，ANF分泌量分别为672±114、794±91和793±125 pg/min（n = 6，P>0.05）。当静脉回流量从2 ml/min增加到5 ml/min时，ANF分泌量从669±81增加到1089±127 pg/min（P<0.01）。向灌注液中添加浓度为50、100或200 pg/ml的AII（每组n = 6）对基础ANF分泌或ANF对静脉回流量增加的反应无显著影响。同样，向灌注液中添加浓度为5、25或100 pg/ml的ADH对心脏释放ANF也无显著影响。这些结果表明，AII和ADH在体内增加血浆ANF浓度的能力可能归因于这些激素对右心房或左心房压力的影响。
