Diagilev V V, Zabezhinskiĭ M A
Vopr Onkol. 1988;34(2):196-9.
The experiments used 144 non-inbred albino male rats aged 2-3 months. They were divided in 6 study groups and one control group. The animals of groups 1-3 received 0.01 ml infusions of 6.5% solution of DMBA into the larynx twice a week for 6 months. In groups 4-5, animals were treated with acetone and dimethylsulfoxide, respectively. Epidermal chalones were instilled in group 2 1 hr after DMBA infusion, and hepatic ones--in group 3. After 7 and 10 months, the animals were sacrificed with large doses of ether, 6 sections were taken from each larynx and stained with hematoxylin--eosin. The statistically treated results showed chalone treatment to inhibit the development of precancerous lesions in larynx induced by DMBA. The greatest inhibitory effect was registered in epidermal chalone treatment.
实验使用了144只2至3个月大的非近交系白化雄性大鼠。它们被分为6个研究组和1个对照组。第1至3组的动物每周两次接受0.01毫升6.5%的DMBA溶液注入喉部，持续6个月。在第4至5组中，动物分别用丙酮和二甲基亚砜进行处理。在DMBA注入后1小时，向第2组注入表皮抑素，向第3组注入肝抑素。7个月和10个月后，用大剂量乙醚处死动物，从每个喉部取6个切片，并用苏木精-伊红染色。经统计学处理的结果表明，抑素治疗可抑制DMBA诱导的喉部癌前病变的发展。表皮抑素治疗的抑制作用最为显著。
