Johne B, Gaudernack G, Mørland B
Institute of Microbiology, Dental Faculty, University of Oslo, Norway.
Acta Pathol Microbiol Immunol Scand C. 1987 Dec;95(6):241-50. doi: 10.1111/j.1699-0463.1987.tb00037.x.
Endotoxins (lipopolysaccharides, LPSs) from the strictly anaerobic bacterium Bacteroides intermedius and from Escherichia coli were compared with respect to effects on human monocytes. Exposure of monocyte monolayer cultures to the structurally different endotoxins caused morphological changes, demonstrated by large rounded cells containing cytoplasmic granules with the B. intermedius LPS, and smaller cells with several cytoplasmic protrusions with the E. coli LPS after 10 days of culture. The B. intermedius LPS was, at some doses, able to induce an increased level of the lysosomal enzyme acid phosphatase and increased C3b-receptor-mediated phagocytosis, while other doses gave no effect or weak suppression compared to control cultures. The stimulatory effects were donor-dependent. The E. coli LPS had predominantly suppressive effects on acid phosphatase level and C3b phagocytosis. Both endotoxins resulted in enhanced expression of the activation related cell surface antigen, characterized by the mononuclear phagocyte specific monoclonal antibody 1D5. Biphasic dose-response relationships were observed, particularly pronounced with B. intermedius LPS, giving stimulation of enzyme activity with low (1 ng/ml) and high (0.5 microgram/ml) doses, and suppressed enzyme activity with intermediate doses (0.01-0.10 microgram/ml). The results imply that the effects of endotoxins on different aspects of human monocytes vary considerably; they also suggest that the balance between stimulatory and suppressive properties of a particular endotoxin may influence its net effect on mononuclear phagocyte functions.
就对人单核细胞的影响而言，对来自严格厌氧细菌中间普氏菌和大肠杆菌的内毒素（脂多糖，LPS）进行了比较。将单核细胞单层培养物暴露于结构不同的内毒素会导致形态变化，培养10天后，可见含有细胞质颗粒的大圆形细胞（中间普氏菌LPS作用下）以及具有几个细胞质突起的较小细胞（大肠杆菌LPS作用下）。在某些剂量下，中间普氏菌LPS能够诱导溶酶体酶酸性磷酸酶水平升高，并增强C3b受体介导的吞噬作用，而与对照培养物相比，其他剂量则无作用或有微弱抑制作用。刺激作用依赖于供体。大肠杆菌LPS对酸性磷酸酶水平和C3b吞噬作用主要具有抑制作用。两种内毒素均导致与活化相关的细胞表面抗原表达增强，其特征为单核吞噬细胞特异性单克隆抗体1D5。观察到双相剂量反应关系，中间普氏菌LPS尤为明显，低剂量（1 ng/ml）和高剂量（0.5 μg/ml）刺激酶活性，中间剂量（0.01 - 0.10 μg/ml）抑制酶活性。结果表明内毒素对人单核细胞不同方面的影响差异很大；这也表明特定内毒素的刺激和抑制特性之间的平衡可能会影响其对单核吞噬细胞功能的净效应。
