Storeng R, Johne B
Acta Pathol Microbiol Immunol Scand B. 1987 Apr;95(2):135-9. doi: 10.1111/j.1699-0463.1987.tb03101.x.
A comparative study on the toxic effects of endotoxin (lipopolysaccharide, LPS) from the strictly anaerobic Bacteroides intermedius BM1 and from Escherichia coli 055 was performed. Pre-implantation mouse embryos at the 2-cell stage from LPS responder (C57BL/6J) and low responder (C3H/Hej) mouse strains were exposed to the endotoxins, and development was observed during 72 h in vitro. Toxic effects of both endotoxins on embryos from both mouse strains were demonstrated. B. intermedius LPS was less toxic than E. coli LPS. The C3H/Hej embryos were more susceptible to endotoxins than the C57BL/6J embryos. A biphasic dose-response relationship was observed, as 100 micrograms/ml LPS was less embryotoxic than 1, 10 or 500 micrograms/ml, suggesting more complex mechanisms of effect than non-specific cell toxicity.
对严格厌氧的中间拟杆菌BM1和大肠杆菌055的内毒素（脂多糖，LPS）的毒性作用进行了比较研究。将来自LPS反应型（C57BL/6J）和低反应型（C3H/Hej）小鼠品系的2细胞期植入前小鼠胚胎暴露于内毒素，并在体外观察72小时内的发育情况。结果表明两种内毒素对两种小鼠品系的胚胎均有毒性作用。中间拟杆菌LPS的毒性低于大肠杆菌LPS。C3H/Hej胚胎比C57BL/6J胚胎对内毒素更敏感。观察到双相剂量反应关系，因为100微克/毫升LPS的胚胎毒性低于1、10或500微克/毫升，这表明其作用机制比非特异性细胞毒性更为复杂。
