Yang Qian, Fang Fei, Li Yue, Ye Yong
J Nanosci Nanotechnol. 2017 Apr;17(4):2394-400. doi: 10.1166/jnn.2017.13436.
The sapogenin isolated from seeds of Camellia oleifera shows central effects in our previous research. Aiming to improve its neuroprotective effects, we synthesize the zinc-sapogenin complex, which has been found with the nanoparticle characters and neuroprotective effects. Its structure is deduced as sapogenin:zinc:water (3:1:1) by ultraviolet spectra, infrared spectra, element analysis, and thermogravimetric analysis. The average size of zinc-sapogenin is 115 nm with plane circular shape by measurement of particle analyzer and scanning electron microscope. The nanoparticle improves DPPH radical scavenging activity due to the enhancement of electron transfer among atoms through the linkage of zinc with the sapogenin. Intraperitoneal injection of zinc-sapogenin reduced mice behavioral disorder and neuron damage induced by neurotoxicity of rotenone, increased antioxidative ability and promoted the neurotransmitters of dopamine and acetylcholine in mice brain. Zinc strengthens stability and effects of the sapogenin, and it is a superior candidate of drugs for neuroprotection without accumulation in brain.
在我们之前的研究中，从油茶种子中分离出的皂素表现出中枢效应。为了提高其神经保护作用，我们合成了锌 - 皂素复合物，发现其具有纳米颗粒特性和神经保护作用。通过紫外光谱、红外光谱、元素分析和热重分析，推断其结构为皂素：锌：水（3:1:1）。通过颗粒分析仪和扫描电子显微镜测量，锌 - 皂素的平均尺寸为115nm，呈平面圆形。由于锌与皂素的连接增强了原子间的电子转移，该纳米颗粒提高了DPPH自由基清除活性。腹腔注射锌 - 皂素可减少鱼藤酮神经毒性诱导的小鼠行为障碍和神经元损伤，提高抗氧化能力，并促进小鼠脑中多巴胺和乙酰胆碱的神经递质分泌。锌增强了皂素的稳定性和作用，并且它是神经保护药物的优良候选者，不会在脑中蓄积。
