从油茶树壳中分离得到一种新型双黄酮类化合物及其自由基清除活性研究

Isolation and free radical scavenging activities of a novel biflavonoid from the shells of Camellia oleifera Abel.

机构信息

Pharmaceutical Engineering Department, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, PR China.

出版信息

Fitoterapia. 2012 Dec;83(8):1585-9. doi: 10.1016/j.fitote.2012.09.006. Epub 2012 Sep 14.

DOI:10.1016/j.fitote.2012.09.006

PMID:22982330

Abstract

Oil production from the seeds of Camellia oleifera Abel. causes a great waste of shells which contain a lot of bioactive components. The aim of this research was to isolate flavonoid from the shells of C. oleifera Abel. and evaluate its uses. The shells were extracted by 70% methanol, hydrolyzed by 2M hydrochloric acid, and further crystallized in acetone, the corresponding yield of flavonoid was up to 2.1% (purity: 93.8%). The procedure is concise, quick and proper for industrial utilization of the shells. Flavonoid was identified as bimolecular kaempferol structure by UV, MS, (1)H NMR and (13)C NMR spectra, which is a new biflavonoid and first found in C. oleifera Abel. It showed stronger scavenging activity of DPPH and ABTS radicals than kaempferol. MDA decreased, and SOD and GSH-Px activities increased significantly in serum (P<0.01) and brain tissue (P<0.05) of mice after intragastric administration of biflavonoid at 200mg/kg/d for 30d. Its effects in vivo are superior to vitamin C and similar to kaempferol. Thus biflavonoid can be used as a prospective antioxidant to protect brain cells against damage from free radicals.

摘要

油茶籽榨油产生大量的废弃果壳，壳中含有许多具有生物活性的成分。本研究旨在从油茶果壳中提取并分离具有生物活性的黄酮类化合物，评估其用途。采用 70%甲醇提取，2M 盐酸水解，丙酮结晶，得到相应的黄酮类化合物，收率为 2.1%（纯度：93.8%）。该方法简洁、快速，适合工业应用。通过紫外光谱、质谱、（1）H NMR 和（13）C NMR 光谱鉴定，该黄酮类化合物为二分子山柰酚结构，为新的二氢黄酮醇类化合物，首次在油茶果壳中发现。该化合物对 DPPH 和 ABTS 自由基的清除能力强于山柰酚。连续灌胃 30d 后，200mg/kg/d 剂量组能显著降低血清和脑组织丙二醛（MDA）水平，提高超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活力（P<0.01）。体内作用优于维生素 C，与山柰酚相当。因此，二氢黄酮醇可作为一种潜在的抗氧化剂，保护脑细胞免受自由基的损伤。