Lin Haiyan, Xing Weijie, Li Yu, Xie Yanxin, Tang Xiaoshi, Zhang Qingxue
a Department of Gynecology & Obstetrics , Sun Yat-sen Memorial Hospital of Sun Yat-sen University , Guangzhou , China.
b Reproductive Center , The Third Affiliated Hospital of Sun Yat-sen University , Guangzhou , China.
Gynecol Endocrinol. 2018 Sep;34(9):784-788. doi: 10.1080/09513590.2018.1459548. Epub 2018 Apr 12.
Polycystic ovary syndrome (PCOS) is a common endocrine disease that affects reproductive-aged women and mostly characterized by insulin resistance (IR). The underlying mechanism remains unknown. Long noncoding RNAs (lncRNAs) have been demonstrated to be involved in various levels of biological regulation process of cell development, metabolism, and differentiation. This study aims to investigate the relationship between IR and differential expression of lncRNA Growth-arrest specific transcript 5 (GAS5) in patients' serum with and without PCOS. A total of 76 cases of serum was collected from non-PCOS and PCOS patients with and without IR to measure interleukin-18 (IL-18) and GAS5 expression, which were correlated with IR status. The IL-18 concentration in serums was significantly increased in PCOS patients with IR. GAS5 expression was decreased in serums in PCOS patients with IR. Result of correlation analysis shows that there is a negative association between GAS5 expression and homeostasis model of assessment for insulin resistance (HOMA-IR). GAS5 was yielded the ROC curve (AUC). Our study implied that elevated IL-18 expression and downregulation of GAS5 in serums might contribute to IR in PCOS patients.
多囊卵巢综合征（PCOS）是一种常见的内分泌疾病，影响育龄妇女，主要特征为胰岛素抵抗（IR）。其潜在机制尚不清楚。长链非编码RNA（lncRNAs）已被证明参与细胞发育、代谢和分化的各级生物调节过程。本研究旨在探讨有无PCOS患者血清中IR与lncRNA生长停滞特异性转录本5（GAS5）差异表达之间的关系。共收集了76例非PCOS和PCOS伴或不伴IR患者的血清，以测量白细胞介素-18（IL-18）和GAS5表达，并将其与IR状态相关联。伴有IR的PCOS患者血清中IL-18浓度显著升高。伴有IR的PCOS患者血清中GAS5表达降低。相关性分析结果显示，GAS5表达与胰岛素抵抗稳态模型评估（HOMA-IR）之间存在负相关。GAS5得出了ROC曲线（AUC）。我们的研究表明，血清中IL-18表达升高和GAS5下调可能导致PCOS患者出现IR。
