Beijing Institute of Radiation Medicine, Beijing 100850, P. R. China.
Analyst. 2018 Apr 30;143(9):2115-2121. doi: 10.1039/c8an00160j.
In accidental irradiation situations, rapid in-field evaluation of acute radiation syndrome is critical for effective triage and timely medical treatment of irradiated individuals. A surface-enhanced Raman scattering (SERS)-based lateral flow assay was developed for the quantitative detection of C-reactive protein (CRP) as an early bio-indicator of a radiation-induced inflammatory response in nonhuman primates. Raman reporter-embedded gold-core silver-shell nanoparticles with built-in hot spots were synthesized and conjugated with a CRP detection antibody to serve as SERS tags in the lateral flow assay. The proposed SERS-based lateral flow assay can rapidly detect CRP with a limit of detection of 0.01 ng mL-1 and quantitative analysis ability. Furthermore, the assay was applied to evaluate the CRP levels in plasma samples of irradiated nonhuman primates at 0 to 80 h after exposure to sublethal (4 Gy) and lethal (8 Gy) doses of total body irradiation (n = 3 animals per group). The plasma CRP levels increase rapidly within few hours after irradiation. The CRP level peaks are observed at 12 or 24 h after irradiation, with a concentration of 201.30, 386.06 and 475.18 μg mL-1 for the 4 Gy irradiated animals and 197.14, 69.52 and 358.03 μg mL-1 for the 8 Gy irradiated animals. The results indicate the potential application of the proposed SERS-based lateral flow assay to serve as a rapid and accurate point-of-care biodosimetry assay for the quantitative detection of bio-indicators to triage irradiated individuals in the field of a radiation accident.
在意外辐射情况下，快速现场评估急性辐射综合征对于有效分类和及时治疗受辐射个体至关重要。本研究开发了一种基于表面增强拉曼散射（SERS）的侧向流动分析，用于定量检测 C 反应蛋白（CRP），作为非人类灵长类动物辐射诱导炎症反应的早期生物标志物。合成了具有内置热点的嵌入拉曼报告器的金核银壳纳米粒子，并将其与 CRP 检测抗体缀合，用作侧向流动分析中的 SERS 标签。所提出的基于 SERS 的侧向流动分析可以快速检测 CRP，检测限为 0.01 ng mL-1，具有定量分析能力。此外，该分析应用于评估暴露于亚致死（4 Gy）和致死（8 Gy）全身照射剂量后 0 至 80 小时的非人类灵长类动物血浆样本中的 CRP 水平（每组 3 只动物）。辐射后数小时内，血浆 CRP 水平迅速升高。在照射后 12 或 24 小时观察到 CRP 水平峰值，4 Gy 照射动物的浓度为 201.30、386.06 和 475.18 μg mL-1，8 Gy 照射动物的浓度为 197.14、69.52 和 358.03 μg mL-1。结果表明，所提出的基于 SERS 的侧向流动分析具有作为快速准确的即时护理生物剂量测定分析的潜力，用于定量检测生物标志物，以对辐射事故现场的受辐射个体进行分类。
