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过表达 OsPGIP2 可通过增强防御机制激活赋予油菜对核盘菌的抗性。

Overexpression of OsPGIP2 confers Sclerotinia sclerotiorum resistance in Brassica napus through increased activation of defense mechanisms.

机构信息

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, China.

Institute of Crop, Wuhan Academy of Agricultural Sciences, Wuhan, Hubei, China.

出版信息

J Exp Bot. 2018 May 25;69(12):3141-3155. doi: 10.1093/jxb/ery138.

DOI:10.1093/jxb/ery138
PMID:29648614
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5972623/
Abstract

Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum, is the most serious disease affecting the yield of the agriculturally and economically important crop Brassica napus (rapeseed). In this study, Oryza sativa polygalacturonase-inhibiting protein 2 (OsPGIP2) was found to effectively enhanced rapeseed immunity against S. sclerotiorum infection. Leaf extracts of B. napus plants overexpressing OsPGIP2 showed enhanced S. sclerotiorum resistance by delaying pathogen infection. The constitutive expression of OsPGIP2 in rapeseed plants provided a rapid and effective defense response, which included the production of reactive oxygen species, interactions with S. sclerotiorum polygalacturonases (SsPG3 and SsPG6), and effects on the expression of defense genes. RNA sequencing analysis revealed that the pathogen induced many differentially expressed genes associated with pathogen recognition, redox homeostasis, mitogen-activated protein kinase signaling cascades, hormone signaling pathways, pathogen-/defense-related genes, and cell wall-related genes. The overexpression of OsPGIP2 also led to constitutively increased cell wall cellulose and hemicellulose contents in stems without compromising seed quality. The results demonstrate that OsPGIP2 plays a major role in rapeseed defense mechanisms, and we propose a model for OsPGIP2-conferred resistance to S. sclerotiorum in these plants.

摘要

菌核病（SSR）由核盘菌引起，是严重影响重要农作物油菜（油菜籽）产量的疾病。在这项研究中，发现水稻多聚半乳糖醛酸酶抑制蛋白 2（OsPGIP2）可有效增强油菜对核盘菌感染的免疫力。过表达 OsPGIP2 的油菜叶片提取物通过延迟病原体感染显示出增强的核盘菌抗性。油菜植物中 OsPGIP2 的组成型表达提供了快速有效的防御反应，包括活性氧的产生、与核盘菌多聚半乳糖醛酸酶（SsPG3 和 SsPG6）的相互作用以及对防御基因表达的影响。RNA 测序分析表明，病原体诱导了许多与病原体识别、氧化还原稳态、丝裂原激活蛋白激酶信号级联、激素信号通路、病原体/防御相关基因和细胞壁相关基因相关的差异表达基因。OsPGIP2 的过表达也导致茎中细胞壁纤维素和半纤维素含量的持续增加，而不影响种子质量。结果表明，OsPGIP2 在油菜防御机制中起主要作用，我们提出了 OsPGIP2 赋予这些植物对核盘菌抗性的模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/8e8031953fa6/ery13806.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/a3ebc8aec88c/ery13801.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/600e863a404f/ery13802.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/e2feabe2fc1e/ery13803.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/60c78f93377d/ery13804.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/5a40431f9acf/ery13805.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/8e8031953fa6/ery13806.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/a3ebc8aec88c/ery13801.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/600e863a404f/ery13802.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/e2feabe2fc1e/ery13803.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/60c78f93377d/ery13804.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/5a40431f9acf/ery13805.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7c5f/5972623/8e8031953fa6/ery13806.jpg

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