State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Synergetic Innovation Center of Food Safety and Nutrition, Shanghai 200436, China; College of Food Science and Technology, Shanghai Ocean University, No 999 Huchenghuan Road, Lingang New District, Shanghai 201306, China.
State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Synergetic Innovation Center of Food Safety and Nutrition, Shanghai 200436, China.
Int J Biol Macromol. 2018 Aug;115:1-9. doi: 10.1016/j.ijbiomac.2018.04.030. Epub 2018 Apr 10.
Improving encapsulation efficacy (EE) and bioavailability of β-galactosidase (β-gal) is always a challenge due to its fragility. In this work, β-gal loaded β-chitosan (CS) nanoparticles (NPs) were successfully prepared based on ionic gelation technique and electrostatic attraction for improving its EE and in vitro releasing capacity. The particle size of β-gal loaded low and high molecular weight (LMW and HMW) β-CS NPs reached 584.37 and 652.46nm, with Zeta-potential (ZP) of 26.37 and 16.46mV under the optimal conditions, respectively. In vitro release study conducted at pH4.5 and 7.4 showed that β-gal loaded LMW and HMW β-CS NPs with EE of 68.32 and 58.64% sustained the release of the β-gal over 12h. The β-gal incorporated into β-CS NPs was confirmed with the results of physicochemical and structural properties of β-gal loaded β-CS NPs, and prepared NPs had hardly any cytotoxicity in the range of 0.1-1.0mg/mL. The results indicated that β-gal loaded β-CS NPs could serve as non-toxic delivery carriers for the treatment of lactose intolerance.
由于 β-半乳糖苷酶（β-gal）的脆弱性，提高其包封效率（EE）和生物利用度一直是一个挑战。在这项工作中，基于离子凝胶技术和静电吸引，成功制备了负载β-半乳糖苷酶的β-壳聚糖（CS）纳米颗粒（NPs），以提高其 EE 和体外释放能力。在最佳条件下，负载低分子量（LMW）和高分子量（HMW）β-CS NPs 的β-gal 的粒径分别达到 584.37nm 和 652.46nm，Zeta 电位（ZP）分别为 26.37mV 和 16.46mV。在 pH4.5 和 7.4 下进行的体外释放研究表明，负载 LMW 和 HMW β-CS NPs 的 EE 分别为 68.32%和 58.64%，β-gal 能够在 12h 内持续释放。负载到 β-CS NPs 中的 β-gal 得到了负载β-CS NPs 的理化和结构特性的证实，并且制备的 NPs 在 0.1-1.0mg/mL 的范围内几乎没有细胞毒性。结果表明，负载β-半乳糖苷酶的β-CS NPs 可以作为治疗乳糖不耐受的无毒递送载体。
