School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China; Laboratory of Aquatic Products Quality & Safety Risk Assessment (Shanghai) at China Ministry of Agriculture, No 999 Huchenghuan Road, Lingang New District, Shanghai, 201306, China; College of Food Science and Technology, Shanghai Ocean University, No 999 Huchenghuan Road, Lingang New District, Shanghai, 201306, China; National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Shanghai, 201306, China.
Laboratory of Aquatic Products Quality & Safety Risk Assessment (Shanghai) at China Ministry of Agriculture, No 999 Huchenghuan Road, Lingang New District, Shanghai, 201306, China; College of Food Science and Technology, Shanghai Ocean University, No 999 Huchenghuan Road, Lingang New District, Shanghai, 201306, China; National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Shanghai, 201306, China.
Carbohydr Polym. 2020 May 15;236:115974. doi: 10.1016/j.carbpol.2020.115974. Epub 2020 Feb 11.
Cellulose nanocrystals (CNCs) were added to lysozyme (Lys) loaded β-chitosan (β-CS) nanoparticles (NPs) based on ionic gelation technique for enhancing their antibacterial stability. Particle size of CNCs stabilized Lys loaded low (L) and high (H) molecular weight (MW) β-CS NPs reached 140.93 and 284.03 nm with encapsulation efficacy (EE) of 59.38 and 51.23 %, respectively. CNCs stabilized Lys loaded LMW β-CS NPs with smaller particle size showed higher antibacterial activity against E. coli and L. innocua than that of LMW β-CS NPs and Lys loaded LMW β-CS NPs. Inhibition zones (IZ) of CNCs stabilized Lys loaded LMW β-CS NPs against E. coli and L. innocua were 13.29 and 15.29 mm, respectively. in vitro release study conducted at pH 4.5 and 7.4 showed that CNCs stabilized Lys loaded β-CS NPs sustained the release of Lys over 12 h. CNCs, as stabilizers and fillers, enhanced and extended antibacterial property of Lys loaded β-CS NPs through electrostatic interaction and colloidal stability. It was demonstrated that CNCs stabilized Lys loaded β-CS NPs could be used as packaging material for shelf life extension.
纤维素纳米晶体（CNCs）被添加到基于离子凝胶技术的溶菌酶（Lys）负载的β-壳聚糖（β-CS）纳米颗粒（NPs）中，以提高其抗菌稳定性。CNCs 稳定的 Lys 负载低（L）和高（H）分子量（MW）β-CS NPs 的粒径分别达到 140.93nm 和 284.03nm，包封效率（EE）分别为 59.38%和 51.23%。粒径较小的 CNCs 稳定的 Lys 负载 LMW β-CS NPs 对大肠杆菌和无害李斯特菌的抗菌活性均高于 LMW β-CS NPs 和 Lys 负载 LMW β-CS NPs。CNCs 稳定的 Lys 负载 LMW β-CS NPs 对大肠杆菌和无害李斯特菌的抑菌圈（IZ）分别为 13.29mm 和 15.29mm。在 pH4.5 和 7.4 下进行的体外释放研究表明，CNCs 稳定的 Lys 负载 β-CS NPs 在 12 小时内持续释放 Lys。CNCs 作为稳定剂和填充物，通过静电相互作用和胶体稳定性增强和延长了 Lys 负载 β-CS NPs 的抗菌性能。结果表明，CNCs 稳定的 Lys 负载 β-CS NPs 可用作延长货架期的包装材料。
