São Paulo State University (UNESP), Department of Chemistry and Biochemistry, School of Technology and Sciences, Presidente Prudente 19060-900, Brazil.
São Paulo State University (UNESP), Department of Chemistry and Biochemistry, School of Technology and Sciences, Presidente Prudente 19060-900, Brazil.
Int J Biol Macromol. 2018 Aug;115:106-113. doi: 10.1016/j.ijbiomac.2018.04.035. Epub 2018 Apr 10.
A fucomannogalactan from Rhizoctonia solani biomass was obtained after hot aqueous extraction and purified by freeze-thaw cycles and gel filtration chromatography on Sepharose CL-6B. The polysaccharide was homogeneous after HPSEC/RID analysis (M/M~1.1), displaying an average molecular weight of 15.4×10Da. Its chemical structure was determined by methylation analysis (GC/MS) and spectroscopy (FTIR, 1D and 2D NMR). The polysaccharide had a branched α-1,6-linked Galp backbone with 66% linear residues, a number of which were at O-3 methylated. Side chains (34%) were always linked at O-2 positions of the main chain and consisted of single, non-reducing ends of α-d-Manp (6%) and α-l-Fucp (28%). Analysis of its biological activity showed that the highly purified fucomannogalactan from R. solani inhibited the proliferation of colon cancer cells in vitro, but that it did not have the same activity against lung cancer cells.
从立枯丝核菌生物质中提取得到一种岩藻甘露半乳聚糖，经过热水提取、冻融循环和 Sepharose CL-6B 凝胶过滤层析进行纯化。经过高效凝胶渗透色谱/折射率检测分析（M/M~1.1），该多糖为均一组分，显示出平均分子量为 15.4×10Da。其化学结构通过甲基化分析（GC/MS）和光谱分析（FTIR、1D 和 2D NMR）确定。该多糖具有分支的α-1,6-连接的 Galp 主链，其中 66%为线性残基，其中一些在 O-3 位被甲基化。侧链（34%）始终连接在主链的 O-2 位置，由单糖、非还原端的α-d-Manp（6%）和α-l-Fucp（28%）组成。对其生物活性的分析表明，从立枯丝核菌中高度纯化的岩藻甘露半乳聚糖能够抑制体外结肠癌细胞的增殖，但对肺癌细胞没有相同的活性。
