Departamento de Química, Universidade Federal de Goiás, Regional Catalão, 75704-020 Catalão, Brazil.
Departamento de Biologia Celular, Universidade Federal do Paraná, 81531-980 Curitiba, Brazil.
Carbohydr Polym. 2019 Dec 1;225:115203. doi: 10.1016/j.carbpol.2019.115203. Epub 2019 Aug 17.
A fucomannogalactan (FMG-Hm), with a molecular weight of 17.1 kDa, obtained from fruiting bodies of Hypsizygus marmoreus exhibited promising in vitro antimelanoma effects. FMG-Hm was not cytotoxic, nor did it alter the cell morphology and proliferation, but was able to inhibit colony-forming ability and cell migration in B16-F10 murine melanoma cells. An analysis of the monosaccharide composition indicated that FMG-Hm was composed of fucose, mannose, and galactose in a ratio of 1.00:1.08:3.17. The FMG-Hm was structurally characterized based on methylation analysis, partial acid hydrolysis, and NMR experiments. The results indicated that FMG-Hm contained a α-(1→6)-linked galactopyranosyl main chain, partially substituted at O-2 by non-reducing ends of α-L-fucopyranose and β-D-mannopyranose. The predicted structure of the heteropolysaccharide was established as.
一种来自玉蕈（Hypsizygus marmoreus）子实体的岩藻半乳聚糖（FMG-Hm），分子量为 17.1 kDa，具有体外抗黑色素瘤的良好效果。FMG-Hm 无细胞毒性，也不改变细胞形态和增殖能力，但能够抑制 B16-F10 鼠黑色素瘤细胞的集落形成能力和细胞迁移。单糖组成分析表明，FMG-Hm 由岩藻糖、甘露糖和半乳糖以 1.00:1.08:3.17 的比例组成。基于甲基化分析、部分酸水解和 NMR 实验对 FMG-Hm 的结构进行了表征。结果表明，FMG-Hm 含有一个 α-(1→6)-连接的半乳糖吡喃糖主链，在 O-2 位被非还原端的α-L-岩藻糖和β-D-甘露糖部分取代。预测的杂多糖结构建立为。
