Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.
Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, PR China.
Int J Biol Macromol. 2015 Feb;73:124-30. doi: 10.1016/j.ijbiomac.2014.10.056. Epub 2014 Nov 20.
A polysaccharide fraction (SHPSA) was obtained from Sargassum horneri by hot-water extraction and sequential purification of anion-exchange chromatography and gel-filtration chromatography. SHPSA was found to be a neutral polysaccharide fraction with an average molecular weight of 5.78×10(5) Da and composed of T-D-Glcp, 1,3-D-Glcp, 1,6-D-Glcp and 1,3,6-D-Glcp in a molar percentage of 1.00:4.17:1.17:0.89, respectively. Based on the results from chemical analysis, NMR, and SHPSA was determined to be a glucan with β-(1→6) side chains linked to a β-(1→3) backbone with relatively few branch points. Moreover, SHPSA could inhibit the growth of human colon cancer DLD cells in a dose-dependent manner by inducing the apoptosis of DLD cells. So, SHPSA was promising for future use as a natural antitumor agent.
从马尾藻中提取多糖（SHPSA），通过热水提取和阴离子交换色谱和凝胶过滤色谱的顺序纯化得到。SHPSA 被发现是一种中性多糖，平均分子量为 5.78×10(5) Da，由 T-D-Glcp、1,3-D-Glcp、1,6-D-Glcp 和 1,3,6-D-Glcp 以摩尔百分比 1.00:4.17:1.17:0.89 组成。基于化学分析、NMR 和 SHPSA 的结果，SHPSA 被确定为一种具有 β-(1→6)侧链连接到 β-(1→3)主链的葡聚糖，分支点相对较少。此外，SHPSA 可以通过诱导 DLD 细胞凋亡，以剂量依赖的方式抑制人结肠癌细胞 DLD 的生长。因此，SHPSA 有望成为未来的天然抗肿瘤药物。
