College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning 110866, China.
College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, Liaoning 110866, China.
Int J Biol Macromol. 2018 Aug;115:45-52. doi: 10.1016/j.ijbiomac.2018.04.039. Epub 2018 Apr 10.
A polysaccharide with a molecular weight of 13,029Da was isolated from Momordica charantia (MCP) fruit and purified by ion-exchange and size-exclusion chromatography. The isolated polysaccharide MCPIIa contained L-Rha, D-GalA, D-Gal, D-Xyl, L-Ara in a molar ratio of 12:3.05:19.89:5.95:56. IR spectrum and NMR studies indicated that the MCPIIa sugar units were linked, via β-glycosidic bonds, to a large number of arabinofuranose, glucuronic acid, and xylopyranosyl residues. In addition, the hypoglycemic effect of MCPIIa was investigated in streptozotocin (STZ)-induced diabetic mice. After STZ-induction, MCPIIa (100, 200, or 300mg/kg body weight) was administered orally, once daily, for 28days. Glycemia in STZ-diabetogenic mice was significantly reduced, and compared with diabetes mellitus (DM) mice, serum insulin concentration increased significantly, following MCPIIa administration. Transmission electron microscopy showed an alleviation of STZ-lesions in pancreatic tissue from mice treated with MCPIIa. These results indicate that MCPIIa may be useful as an anti-diabetic agent.
从苦瓜（MCP）果实中分离出一种分子量为 13029Da 的多糖，并通过离子交换和分子筛层析进行纯化。分离得到的多糖 MCPIIa 含有 L-Rha、D-GalA、D-Gal、D-Xyl、L-Ara，摩尔比为 12:3.05:19.89:5.95:56。IR 光谱和 NMR 研究表明，MCPIIa 的糖单元通过β-糖苷键与大量阿拉伯呋喃糖、葡萄糖醛酸和木吡喃糖残基相连。此外，还研究了 MCPIIa 在链脲佐菌素（STZ）诱导的糖尿病小鼠中的降血糖作用。STZ 诱导后，MCPIIa（100、200 或 300mg/kg 体重）每天口服一次，共 28 天。与糖尿病（DM）小鼠相比，STZ 致糖尿病小鼠的血糖明显降低，给予 MCPIIa 后血清胰岛素浓度显著升高。透射电镜显示 MCPIIa 处理的小鼠胰腺组织中的 STZ 损伤得到缓解。这些结果表明，MCPIIa 可用作抗糖尿病药物。
