Aipire Adila, Yuan Pengfei, Aimaier Alimu, Cai Shanshan, Mahabati Mahepali, Lu Jun, Ying Tianlei, Zhang Baohong, Li Jinyao
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.
School of Science, and School of Interprofessional Health Studies, Faculty of Health & Environmental Sciences, Auckland University of Technology, Auckland 1142, New Zealand.
Biomolecules. 2020 Jan 19;10(1):159. doi: 10.3390/biom10010159.
is a Chinese herbal medicine with various bioactivities. Three fractions (GUPS-I, GUPS-II and GUPS-III) of polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively. The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different. The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages. Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I. An in vivo experiment showed that only GUPS-II significantly enhanced the maturation of DCs. These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.
是一种具有多种生物活性的中草药。获得了多糖（GUPS）的三个级分（GUPS-I、GUPS-II和GUPS-III），分子量分别为1.06、29.1和14.9 kDa。GUPS-II和GUPS-III的单糖组成相似，而GUPS-I的单糖组成明显不同。扫描电子显微镜、傅里叶变换红外光谱和核磁共振的结果表明，GUPS-II和GUPS-III呈片状，表面光滑，含有α-和β-糖苷键，而GUPS-I呈颗粒状，仅含有α-糖苷键。此外，在体外，GUPS-II和GUPS-III对树突状细胞（DCs）的成熟和细胞因子产生表现出比GUPS-I更好的生物活性。体内实验表明，只有GUPS-II显著增强了DCs的成熟。这些结果表明，GUPS-II有潜力与癌症免疫疗法联合使用以提高治疗效果。
