Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), University of Palermo, Viale delle Scienze, Ed. 17, 90128 Palermo, Italy.
Dipartimento di Fisica e Chimica (DiFC), University of Palermo, Viale delle Scienze, Ed. 17, 90128 Palermo, Italy.
J Colloid Interface Sci. 2018 Aug 15;524:156-164. doi: 10.1016/j.jcis.2018.04.025. Epub 2018 Apr 6.
Oral insulin administration is not actually effective due to insulin rapid degradation, inactivation and digestion by proteolytic enzymes which results in low bioavailability. Moreover insulin is poorly permeable and lack of lipophilicity. These limits can be overcome by the loading of protein in some nanostructured carrier such as halloysite nanotubes (HNTs).
Herein we propose an easy strategy to obtain HNT hybrid materials for the delivery of insulin. We report a detailed description on the thermal behavior and stability of insulin loaded and released from the HNTs hybrid by the combination of several techniques.
Release experiments of insulin from the HNTs revealed the efficacy of the nanocarrier. Circular Dichroism data evidenced that the released insulin exhibits its native-like secondary structure confirming the suitability of HNT/insulin as delivery system for at least three months. The loaded nanotubes were filled into chitosan matrix with the aim to prepare bionanocomposite films that can be used for transdermal delivery. This work puts forward an efficient strategy to prepare halloysite based nanocarriers containing insulin that could be employed in several biomedical applications. The detailed description of the prepared HNT/insulin hybrid represents a fundamental point for designing advanced delivery systems.
由于胰岛素会被蛋白酶快速降解、失活和消化，导致生物利用度低，因此口服胰岛素给药实际上并不有效。此外，胰岛素的渗透性差且缺乏亲脂性。这些限制可以通过将蛋白质加载到某些纳米结构载体（如埃洛石纳米管（HNTs））中来克服。
本文提出了一种简单的策略，用于获得用于递送胰岛素的 HNT 杂化材料。我们报告了详细描述了通过几种技术的组合，负载和从 HNTs 杂化材料中释放胰岛素的热行为和稳定性。
从 HNTs 中释放胰岛素的实验证明了纳米载体的功效。圆二色性数据表明，释放的胰岛素表现出其天然样二级结构，证实了 HNT/胰岛素作为至少三个月的递送系统的适用性。将负载的纳米管填充到壳聚糖基质中，目的是制备可用于透皮递送的生物纳米复合材料薄膜。这项工作提出了一种有效的策略来制备含有胰岛素的基于埃洛石的纳米载体，这些载体可用于多种生物医学应用。对制备的 HNT/胰岛素杂化物的详细描述代表了设计先进递送系统的重要基础。
