Department of Oceanography, Daniel K. Inouye Center for Microbial Oceanography: Research and Education, University of Hawai'i at Mānoa, 1950 East-West Road, Honolulu, HI 96822, United States.
Department of Oceanography, Daniel K. Inouye Center for Microbial Oceanography: Research and Education, University of Hawai'i at Mānoa, 1950 East-West Road, Honolulu, HI 96822, United States.
Virology. 2018 May;518:423-433. doi: 10.1016/j.virol.2018.03.010. Epub 2018 Apr 9.
The family Mimiviridae contains uncommonly large viruses, many of which were isolated using a free-living amoeba as a host. Although the genomes of these and other mimivirids that infect marine heterokont and haptophyte protists have now been sequenced, there has yet to be a genomic investigation of a mimivirid that infects a member of the Viridiplantae lineage (green algae and land plants). Here we characterize the 668-kilobase complete genome of TetV-1, a mimivirid that infects the cosmopolitan green alga Tetraselmis (Chlorodendrophyceae). The analysis revealed genes not previously seen in viruses, such as the mannitol metabolism enzyme mannitol 1-phosphate dehydrogenase, the saccharide degradation enzyme alpha-galactosidase, and the key fermentation genes pyruvate formate-lyase and pyruvate formate-lyase activating enzyme. The TetV genome is the largest sequenced to date for a virus that infects a photosynthetic organism, and its genes reveal unprecedented mechanisms by which viruses manipulate their host's metabolism.
Mimiviridae 科家族包含非常大的病毒，其中许多病毒是使用自由生活的变形虫作为宿主分离出来的。虽然这些病毒以及其他感染海洋异鞭毛体和甲藻原生生物的 mimivirid 的基因组已经被测序，但尚未对感染 Viridiplantae 谱系（绿藻和陆地植物）成员的 mimivirid 进行基因组研究。在这里，我们描述了感染全球分布的绿藻 Tetraselmis（Chlorodendrophyceae）的 mimivirid TetV-1 的 668 千碱基完整基因组。分析显示了以前在病毒中未见过的基因，例如甘露醇代谢酶甘露醇 1-磷酸脱氢酶、糖降解酶 alpha-半乳糖苷酶以及关键发酵基因丙酮酸甲酸裂解酶和丙酮酸甲酸裂解酶激活酶。TetV 基因组是迄今为止测序的最大的感染光合生物的病毒基因组，其基因揭示了病毒操纵宿主代谢的前所未有的机制。
