用 Vermamoeba vermiformis 共培养分离出 Yasminevirus，这是 Klosneuvirinae 目中第一个被分离的病毒，显示出扩展的翻译装置组件库。
Isolation of Yasminevirus, the First Member of Klosneuvirinae Isolated in Coculture with Vermamoeba vermiformis, Demonstrates an Extended Arsenal of Translational Apparatus Components.
机构信息
Biochemistry Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
出版信息
J Virol. 2019 Dec 12;94(1). doi: 10.1128/JVI.01534-19.
The family of giant viruses is still expanding, and evidence of a translational machinery is emerging in the virosphere. The Klosneuvirinae group of giant viruses was first reconstructed from studies, and then a unique member was isolated, Bodo saltans virus. Here we describe the isolation of a new member in this group using coculture with the free-living amoeba This giant virus, called Yasminevirus, has a 2.1-Mb linear double-stranded DNA genome encoding 1,541 candidate proteins, with a GC content estimated at 40.2%. Yasminevirus possesses a nearly complete translational machinery, with a set of 70 tRNAs associated with 45 codons and recognizing 20 amino acids (aa), 20 aminoacyl-tRNA synthetases (aaRSs) recognizing 20 aa, as well as several translation factors and elongation factors. At the genome scale, evolutionary analyses placed this virus in the Klosneuvirinae group of giant viruses. Rhizome analysis demonstrated that the genome of Yasminevirus is mosaic, with ∼34% of genes having their closest homologues in other viruses, followed by ∼13.2% in , ∼7.2% in , and less than 1% in Among giant virus sequences, Yasminevirus shared 87% of viral hits with Klosneuvirinae. This description of Yasminevirus sheds light on the Klosneuvirinae group in a captivating quest to understand the evolution and diversity of giant viruses. Yasminevirus is an icosahedral double-stranded DNA virus isolated from sewage water by amoeba coculture. Here its structure and replicative cycle in the amoeba are described and genomic and evolutionary studies are reported. This virus belongs to the Klosneuvirinae group of giant viruses, representing the second isolated and cultivated giant virus in this group, and is the first isolated using a coculture procedure. Extended translational machinery pointed to Yasminevirus among the quasiautonomous giant viruses with the most complete translational apparatus of the known virosphere.
巨型病毒家族仍在不断扩张，病毒圈内不断出现翻译机制的证据。巨型病毒 Klosneuvirinae 组最初是通过研究重建的，然后分离出一个独特的成员，即 Bodo saltans 病毒。在这里，我们描述了使用自由生活的变形虫共培养分离该组中一个新成员的方法。这种名为 Yasminevirus 的巨型病毒具有 2.1-Mb 的线性双链 DNA 基因组，编码 1541 个候选蛋白，GC 含量估计为 40.2%。Yasminevirus 拥有几乎完整的翻译机制，包括一套 70 个 tRNA，与 45 个密码子和识别 20 种氨基酸（aa）相关联，20 种氨酰-tRNA 合成酶（aaRS）识别 20 种 aa，以及几种翻译因子和延伸因子。在基因组规模上，进化分析将这种病毒置于巨型病毒的 Klosneuvirinae 组中。根状茎分析表明，Yasminevirus 的基因组是镶嵌的，约 34%的基因与其他病毒的最近同源物相似，其次是与 相似的约 13.2%，与 相似的约 7.2%，与 相似的不到 1%。在巨型病毒序列中，Yasminevirus 与 Klosneuvirinae 的病毒相似度为 87%。对 Yasminevirus 的描述揭示了 Klosneuvirinae 组在探索巨型病毒进化和多样性方面的迷人探索。Yasminevirus 是一种通过变形虫共培养从污水中分离出的二十面体双链 DNA 病毒。本文描述了该病毒在变形虫中的结构和复制周期，并报告了基因组和进化研究。这种病毒属于巨型病毒的 Klosneuvirinae 组，是该组中第二个被分离和培养的巨型病毒，也是第一个使用共培养程序分离的病毒。扩展的翻译机制将 Yasminevirus 归类为具有最完整翻译装置的准自主巨型病毒之一，是已知病毒界中最完整的翻译装置之一。
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