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比较分析来自同一遗传背景的小鼠胚胎干细胞中的原始态、诱导态和基础态多能性。

Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background.

机构信息

Ghent-Fertility and Stem Cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, Corneel Heymanslaan 10, 9000, Ghent, Belgium.

Department of Anatomy and Embryology, Leiden University Medical Center, Einthovenweg 20, 2333 ZC, Leiden, The Netherlands.

出版信息

Sci Rep. 2018 Apr 12;8(1):5884. doi: 10.1038/s41598-018-24051-5.

DOI:10.1038/s41598-018-24051-5
PMID:29650979
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5897387/
Abstract

Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency.

摘要

小鼠胚胎干细胞（mESCs）存在于原始态、起始态和多能性基础态。虽然已经报道了对这些多能性状态的比较分析，但所使用的 mESC 源自不同的遗传背景，并在不同的实验室中获得。在传统的 LIF+血清培养条件下，mESC 的诱导取决于品系，不同的遗传背景可能会影响后续的干细胞特性。在本研究中，我们通过比较转录谱，对源自我们实验室同一遗传背景的原始态、起始态和多能性基础态 mESC 进行了全面表征。我们展示了原始态、起始态和多能性基础态 mESC 的独特转录谱。虽然原始态和多能性基础态 mESC 具有更相似但不完全相同的谱，但起始态 mESC 显示出非常独特的谱。我们进一步证明，mESC 向特定胚层的分化倾向高度依赖于它们各自的多能性状态。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/a03e165c8b4a/41598_2018_24051_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/d1dced50f257/41598_2018_24051_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/4f0400a12d97/41598_2018_24051_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/0f44bff014f8/41598_2018_24051_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/1f692f3d7b4d/41598_2018_24051_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/48bb3dcc8916/41598_2018_24051_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/8227211eeb5f/41598_2018_24051_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/a03e165c8b4a/41598_2018_24051_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/d1dced50f257/41598_2018_24051_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/4f0400a12d97/41598_2018_24051_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/0f44bff014f8/41598_2018_24051_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/1f692f3d7b4d/41598_2018_24051_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/48bb3dcc8916/41598_2018_24051_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/8227211eeb5f/41598_2018_24051_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cb07/5897387/a03e165c8b4a/41598_2018_24051_Fig7_HTML.jpg

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