Gomer C J, Rucker N, Murphree A L
Clayton Ocular Oncology Center, Children's Hospital of Los Angeles, California 90027.
Int J Radiat Biol Relat Stud Phys Chem Med. 1988 Apr;53(4):651-9. doi: 10.1080/09553008814550971.
The transformation and mutagenic potential of porphyrin photodynamic therapy has been examined in mammalian cells. The mutagenic frequency in Chinese hamster cells at the Na+/K+ ATPase locus was measured by resistance to ouabain following treatment with either photodynamic therapy (PDT) or UV irradiation. The C3H 10T 1/2 mouse embryo cell system was used to document the transformation frequency following PDT, UV irradiation, gamma irradiation or exposure to 3-methylcholanthrene (MCA). Treatments with UV irradiation were effective in producing mutants resistant to ouabain, and treatments with UV irradiation, gamma irradiation and MCA generated transformants at frequencies comparable to those which are reported in the literature. However, PDT treatment conditions (which produced a full range of cytotoxicity) did not induce any mutagenic or transformation activity above background levels.
已在哺乳动物细胞中研究了卟啉光动力疗法的转化和诱变潜力。在用光动力疗法（PDT）或紫外线照射处理后，通过对哇巴因的抗性来测量中国仓鼠细胞在Na+/K+ ATP酶位点的诱变频率。使用C3H 10T 1/2小鼠胚胎细胞系统来记录PDT、紫外线照射、γ射线照射或暴露于3-甲基胆蒽（MCA）后的转化频率。紫外线照射处理有效地产生了对哇巴因有抗性的突变体，并且紫外线照射、γ射线照射和MCA处理产生转化体的频率与文献报道的频率相当。然而，PDT处理条件（产生了一系列细胞毒性）并未诱导出高于背景水平的任何诱变或转化活性。
