Rajakumar Thangarasu, Pugalendhi Pachaiappan, Thilagavathi Subbaiyan
Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Chidambaram, 608002 Tamilnadu India.
Indian J Clin Biochem. 2018 Apr;33(2):171-177. doi: 10.1007/s12291-017-0663-4. Epub 2017 May 13.
The present study aimed to investigate the protective effect of allyl isothiocyanate (AITC) on glycoprotein components in 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis in female Sprague-Dawley rats. Mammary tumor was induced by a single dose of DMBA (25 mg/rat) injected subcutaneously near mammary gland. The levels of glycoprotein components such as hexose, hexosamine and sialic acid were analyzed colorimetrically in plasma, mammary and liver tissues. We observed an increased levels of glycoprotein components in plasma, mammary and liver tissues in cancer bearing rats. It was further confirmed by Periodic Acid Schiff staining in mammary and liver tissues. Upon oral administration of AITC to DMBA injected rats, the abnormal changes were reverted back to near normal levels and biochemical findings are supported by histological analysis. This could be due to the anti-neoplastic potential of AITC against DMBA-induced mammary carcinogenesis. The result shows that AITC has the potential to inhibit abnormal glycosylation that favors neoplastic transformation.
本研究旨在探讨异硫氰酸烯丙酯（AITC）对7,12-二甲基苯并(a)蒽（DMBA）诱导的雌性Sprague-Dawley大鼠乳腺癌变过程中糖蛋白成分的保护作用。通过在乳腺附近皮下注射单剂量的DMBA（25 mg/大鼠）诱导乳腺肿瘤。采用比色法分析血浆、乳腺和肝脏组织中糖蛋白成分（如己糖、氨基己糖和唾液酸）的水平。我们观察到荷癌大鼠血浆、乳腺和肝脏组织中糖蛋白成分水平升高。这在乳腺和肝脏组织的过碘酸希夫染色中得到进一步证实。给注射了DMBA的大鼠口服AITC后，异常变化恢复到接近正常水平，组织学分析也支持生化结果。这可能是由于AITC对DMBA诱导的乳腺癌变具有抗肿瘤潜力。结果表明，AITC有潜力抑制有利于肿瘤转化的异常糖基化。
