Liao Chong-Yu, Feng Ying-Cai, Li Gang, Shen Xiao-Min, Liu Shi-Huo, Dou Wei, Wang Jin-Jun
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China.
Front Physiol. 2018 Mar 29;9:314. doi: 10.3389/fphys.2018.00314. eCollection 2018.
The citrus red mite, , a major citrus pest distributed worldwide, has evolved severe resistance to various classes of chemical acaricides/insecticides including pyrethroids. It is well known that the resistance to pyrethroids is mainly caused by point mutations of voltage-gated sodium channel gene in a wide range of pests. However, increasing number of evidences support that pyrethroids resistance might also be resulted from the integrated mechanisms including metabolic mechanisms. In this study, firstly, comparative analysis of RNA-seq data showed that multiple detoxification genes, including a GSTs gene , were up-regulated in a fenpropathrin-resistant population compared with the susceptible strain (SS). Quantitative real time-PCR results showed that the exposure of fenpropathrin had an induction effect on the transcription of in a time-dependent manner. inhibition and metabolic assay of recombinant PcGSTd1 found that fenpropathrin might not be metabolized directly by this protein. However, its antioxidant role in alleviating the oxidative stress caused by fenpropathrin was demonstrated via the reversely genetic experiment. Our results provide a list of candidate genes which may contribute to a multiple metabolic mechanisms implicated in the evolution of fenpropathrin resistance in the field population of . Furthermore, during the detoxification process, plays an antioxidant role by detoxifying lipid peroxidation products induced by fenpropathrin.
柑橘全爪螨是一种分布于全球的主要柑橘害虫，已对包括拟除虫菊酯在内的各类化学杀螨剂/杀虫剂产生了严重抗性。众所周知，在多种害虫中，对拟除虫菊酯的抗性主要由电压门控钠通道基因的点突变引起。然而，越来越多的证据支持，拟除虫菊酯抗性也可能源于包括代谢机制在内的综合机制。在本研究中，首先，RNA测序数据的比较分析表明，与敏感品系（SS）相比，在甲氰菊酯抗性种群中多个解毒基因（包括一个谷胱甘肽S-转移酶基因）上调。实时定量PCR结果表明，甲氰菊酯暴露对该基因的转录具有时间依赖性诱导作用。重组PcGSTd1的抑制和代谢试验发现，甲氰菊酯可能不会被该蛋白直接代谢。然而，通过反向遗传实验证明了其在减轻甲氰菊酯引起的氧化应激方面的抗氧化作用。我们的结果提供了一份候选基因清单，这些基因可能参与了柑橘全爪螨田间种群对甲氰菊酯抗性进化过程中的多种代谢机制。此外，在解毒过程中，该基因通过解毒甲氰菊酯诱导的脂质过氧化产物发挥抗氧化作用。
