Shang Yi, Wang Yanbo, Deng Jianyu, Liu Xunyue, Fang Yihao, Rao Qiong, Wu Huiming
College of Agriculture and Food Sciences, Zhejiang A&F University, Hangzhou 311300, China.
Kaihua County Agro-Tech Extension and Service Center, Quzhou 324300, China.
Insects. 2020 Oct 26;11(11):730. doi: 10.3390/insects11110730.
The use of a large number of chemical acaricides to control these pest mites has led to an increasing problem of pesticide resistance, which has always been the difficulty in integrated pest management (IPM). Fluazinam has a good control effect on , the serious pest on citrus; however, we only know the mechanism of action of fluazinam as a fungicide and its mechanism of action on mites remains unclear. Through analysis using Illumina high-throughput transcriptomic sequencing and differential expression genes in treated with fluazinam, 59 cytochrome P450 genes, 23 glutathione s-transferase genes, five carboxylate esterase genes, 11 superoxide dismutase genes and 15 catalase genes were identified. The Gene Ontology enrichment and the enrichment of KEGG results showed that the treatment were enrichment for redox enzyme pathways. Evaluating the efficacy of fluazinam, and analyzing the transcriptome data of under fluazinam stress, potentially provide a new agent for prevention and control of , and also preliminary research results for exploring the mechanism of action of fluazinam on . Given the up-regulated expression levels of genes for Mn-superoxide dismutase and catalase, we speculate that they play an important role in fluazinam-stress action on
使用大量化学杀螨剂来控制这些害虫螨已导致农药抗性问题日益严重，这一直是有害生物综合治理（IPM）中的难题。氟唑菌酰胺对柑橘上的严重害虫有很好的防治效果；然而，我们只知道氟唑菌酰胺作为杀菌剂的作用机制，其对螨的作用机制仍不清楚。通过使用Illumina高通量转录组测序和对经氟唑菌酰胺处理的[具体对象未明确]进行差异表达基因分析，鉴定出59个细胞色素P450基因、23个谷胱甘肽S -转移酶基因、5个羧酸酯酶基因、11个超氧化物歧化酶基因和15个过氧化氢酶基因。基因本体论富集和KEGG结果的富集表明，处理后的样本在氧化还原酶途径上有富集。评估氟唑菌酰胺的功效，并分析氟唑菌酰胺胁迫下[具体对象未明确]的转录组数据，可能为[具体对象未明确]的防治提供一种新药剂，也为探索氟唑菌酰胺对[具体对象未明确]的作用机制提供初步研究结果。鉴于锰超氧化物歧化酶和过氧化氢酶基因的表达水平上调，我们推测它们在氟唑菌酰胺对[具体对象未明确]的胁迫作用中起重要作用 。
