Chu Thi Thu Huyen, Hoang Thi Giang, Trinh Duy Chi, Bureau Charlotte, Meynard Donaldo, Vernet Aurore, Ingouff Mathieu, Do Nang Vinh, Périn Christophe, Guiderdoni Emmanuel, Gantet Pascal, Maurel Christophe, Luu Doan-Trung
BPMP, Univ Montpellier, CNRS, INRA, SupAgro, Montpellier, France.
University of Science and Technology of Hanoi, LMI RICE, 18 Hoang Quoc Viet, Nghia Do, Cau Giay, Hanoi, Vietnam.
Rice (N Y). 2018 Apr 12;11(1):23. doi: 10.1186/s12284-018-0209-2.
Cell biology approach using membrane protein markers tagged with fluorescent proteins highlights the dynamic behaviour of plant cell membranes, not only in the standard but also in changing environmental conditions. In the past, this strategy has been extensively developed in plant models such as Arabidopsis.
Here, we generated a set of transgenic lines expressing membrane protein markers to extend this approach to rice, one of the most cultivated crop in the world and an emerging plant model. Lines expressing individually eight membrane protein markers including five aquaporins (OsPIP1;1, OsPIP2;4, OsPIP2;5, OsTIP1;1, OsTIP2;2) and three endosomal trafficking proteins (OsRab5a, OsGAP1, OsSCAMP1) were obtained. Importantly, we challenged in roots the aquaporin-expressing transgenic lines upon salt and osmotic stress and uncovered a highly dynamic behaviour of cell membrane.
We have uncovered the relocalization and dynamics of plasma membrane aquaporins upon salt and osmotic stresses in rice. Importantly, our data support a model where relocalization of OsPIPs is concomitant with their high cycling dynamics.
使用荧光蛋白标记的膜蛋白标记物的细胞生物学方法不仅能突出植物细胞膜在标准条件下，也能突出在变化的环境条件下的动态行为。过去，这种策略已在诸如拟南芥等植物模型中得到广泛发展。
在此，我们构建了一组表达膜蛋白标记物的转基因株系，将这种方法扩展到水稻，水稻是世界上种植最广泛的作物之一，也是一种新兴的植物模型。我们获得了分别表达八种膜蛋白标记物的株系，其中包括五种水通道蛋白（OsPIP1;1、OsPIP2;4、OsPIP2;5、OsTIP1;1、OsTIP2;2）和三种内体运输蛋白（OsRab5a、OsGAP1、OsSCAMP1）。重要的是，我们在盐胁迫和渗透胁迫下对表达水通道蛋白的转基因株系的根部进行了研究，发现了细胞膜的高度动态行为。
我们揭示了水稻在盐胁迫和渗透胁迫下质膜水通道蛋白的重新定位和动态变化。重要的是，我们的数据支持了一个模型，即OsPIPs的重新定位与其高循环动态是相伴发生的。
