Tobacco Research Institute of Chinese Academy of Agricultural Sciences (CAAS) , Qingdao 266101 , PR China.
J Agric Food Chem. 2018 May 2;66(17):4531-4540. doi: 10.1021/acs.jafc.8b00285. Epub 2018 Apr 18.
The enantioselective bioaccumulation and toxicity of dinotefuran in earthworms were studied in this study. The results showed that S-dinotefuran accumulated faster than Rac-dinotefuran and R-dinotefuran in earthworms. The acute toxicity of S-dinotefuran was 1.49 and 2.67 times that of the Rac-dinotefuran and R-dinotefuran in artificial soil during 14 days of exposure. At 1.0 mg/kg, the three tested chemicals inhibited the growth and reproduction as well as induced oxidative stress effects in earthworms; however, the toxic effects induced by S-dinotefuran were the most serious. The transcriptome sequencing results showed that S-dinotefuran had stronger interactions to biomacromolecules and influences on the endoplasmic reticulum (ER) than R-dinotefuran, which may be the main reason for enantioselectivities between the two enantiomers. The present results indicated that the risk of S-dinotefuran was higher than that of Rac-dinotefuran and R-dinotefuran in the soil environment to earthworms. Risk assessment of dinotefuran should be evaluated at the enantiomer level.
本研究探讨了呋虫胺对蚯蚓的对映体选择性生物累积和毒性。结果表明，S-呋虫胺在蚯蚓体内的积累速度快于 Rac-呋虫胺和 R-呋虫胺。在 14 天的暴露期内，S-呋虫胺在人工土壤中的急性毒性分别是 Rac-呋虫胺和 R-呋虫胺的 1.49 倍和 2.67 倍。在 1.0mg/kg 时，这三种受试化学物质均抑制了蚯蚓的生长和繁殖，并诱导了氧化应激效应；然而，S-呋虫胺引起的毒性效应最为严重。转录组测序结果表明，S-呋虫胺与生物大分子的相互作用以及对内质网（ER）的影响均强于 R-呋虫胺，这可能是两种对映体之间对映选择性的主要原因。本研究结果表明，S-呋虫胺在土壤环境中对蚯蚓的风险高于 Rac-呋虫胺和 R-呋虫胺。应对呋虫胺进行对映体水平的风险评估。
