College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
Tobacco Research Institute of Chinese Academy of Agricultural Sciences (CAAS), Qingdao 266101, China.
Molecules. 2018 Nov 16;23(11):2995. doi: 10.3390/molecules23112995.
A great deal of attention has been focused on the secondary metabolites produced by marine endophytic fungi, which can be better alternatives to chemicals, such as biopesticides, for control of polyphagous pests. On the basis of its novel biocontrol attributes, chemical investigation of a marine alga-derived endophytic fungus, , resulted in the isolation of three chloramphenicol derivatives (compounds ⁻). Their chemical structures were elucidated by detailed analysis of their nuclear magnetic resonance spectra, high-resolution electrospray ionization mass spectrometry, and by comparison with the data available in the literature. In this paper, compound was firstly reported as the natural origin of these fungal secondary metabolites. The insecticidal activities of compounds ⁻ against the cotton bollworm, , were evaluated. The natural compound presented considerable activity against , with an LC value of 0.56 ± 0.03 mg/mL (compared to matrine with an LC value of 0.24 ± 0.01 mg/mL). Transcriptome sequencing was used to evaluate the molecular mechanism of the insecticidal activities. The results presented in this study should be useful for developing compound as a novel, ecofriendly and safe biopesticide.
人们高度关注海洋内生真菌产生的次生代谢产物，它们可以作为农药的替代品，特别是用于防治多食性害虫的生物农药。基于其新颖的生物防治特性，对海洋藻类内生真菌 进行了化学研究，从中分离得到了三种氯霉素衍生物（化合物 ⁻ ）。通过详细分析其核磁共振谱、高分辨电喷雾电离质谱，并与文献中的数据进行比较，确定了它们的化学结构。在本文中，首次报道了化合物 是这些真菌次生代谢产物的天然来源。评估了化合物 ⁻ 对棉铃虫 的杀虫活性。天然化合物 对 表现出相当大的活性，LC 值为 0.56 ± 0.03 mg/mL（与苦参碱的 LC 值 0.24 ± 0.01 mg/mL 相比）。通过转录组测序评估了杀虫活性的分子机制。本研究的结果对于开发化合物 作为新型、环保和安全的生物农药应该是有用的。
