Opt Lett. 2018 Apr 15;43(8):1870-1873. doi: 10.1364/OL.43.001870.
We propose a numerical and totally automatic phase aberration compensation method in digital holographic microscopy. The phase aberrations are extracted in a nonlinear optimization procedure in which the phase variation of the reconstructed object wave is minimized. Not only phase curvature but also high-order aberrations could be corrected without extra devices. The correction is directly carried out with the wrapped phase map, which is not affected by phase unwrapping or fitting errors. Numerical simulation proves that the proposed method is more accurate than the conventional surface fitting method without selecting a cell-free background. Experimental results demonstrate the availability of the proposed method in real-time analysis of living cells.
我们提出了一种数字全自动化的相位差补偿方法，应用于数字全息显微镜中。该方法通过非线性优化程序提取相位差，使重建物体波的相位变化最小化。该方法不仅可以校正相位曲率，还可以校正高阶像差，而无需额外的设备。校正可以直接在包裹相位图上进行，而不受相位解包裹或拟合误差的影响。数值模拟证明，与传统的表面拟合方法相比，该方法在不选择无细胞背景的情况下更为准确。实验结果表明，该方法在实时分析活细胞方面具有有效性。
