Kemper Björn, Kosmeier Sebastian, Langehanenberg Patrik, von Bally Gert, Bredebusch Ilona, Domschke Wolfram, Schnekenburger Jürgen
University of Muenster, Laboratory of Biophysics, Robert-Koch-Str. 45, D-48129 Muenster, Germany.
J Biomed Opt. 2007 Sep-Oct;12(5):054009. doi: 10.1117/1.2798639.
A method for the determination of the integral refractive index of living cells in suspension by digital holographic microscopy is described. Digital holographic phase contrast images of spherical cells in suspension are recorded, and the radius as well as the integral refractive index are determined by fitting the relation between cell thickness and phase distribution to the measured phase data. The algorithm only requires information about the refractive index of the suspension medium and the image scale of the microscope system. The specific digital holographic microscopy advantage of subsequent focus correction allows a simultaneous investigation of cells in different focus planes. Results obtained from human pancreas and liver tumor cells show that the integral cellular refractive index decreases with increasing cell radius.
本文描述了一种通过数字全息显微镜测定悬浮液中活细胞积分折射率的方法。记录悬浮液中球形细胞的数字全息相衬图像，并通过将细胞厚度与相位分布之间的关系拟合到测量的相位数据来确定细胞半径和积分折射率。该算法仅需要有关悬浮介质折射率和显微镜系统图像比例的信息。后续焦点校正的数字全息显微镜的特定优势允许同时研究不同焦平面中的细胞。从人胰腺和肝癌细胞获得的结果表明，细胞的积分折射率随着细胞半径的增加而降低。
