Laboratory for Computational Sensing and Robotics, The Johns Hopkins University, Baltimore, Maryland.
The Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins Medical Institutions, Baltimore, Maryland.
J Biophotonics. 2018 Sep;11(9):e201800021. doi: 10.1002/jbio.201800021. Epub 2018 Jun 28.
A sensitive, noninvasive method to detect localized prostate cancer, particularly for early detection and repetitive study in patients undergoing active surveillance, remains an unmet need. Here, we propose a molecular photoacoustic (PA) imaging approach by targeting the prostate-specific membrane antigen (PSMA), which is over-expressed in the vast majority of prostate cancers. We performed spectroscopic PA imaging in an experimental model of prostate cancer, namely, in immunocompromised mice bearing PSMA+ (PC3 PIP) and PSMA- (PC3 flu) tumors through administration of the known PSMA-targeted fluorescence agent, YC-27. Differences in contrast between PSMA+ and isogenic control tumors were observed upon PA imaging, with PSMA+ tumors showing higher contrast in average of 66.07-fold with 5 mice at the 24-hour postinjection time points. These results were corroborated using standard near-infrared fluorescence imaging with YC-27, and the squared correlation between PA and fluorescence intensities was 0.89. Spectroscopic PA imaging is a new molecular imaging modality with sufficient sensitivity for targeting PSMA in vivo, demonstrating the potential applications for other saturable targets relevant to cancer and other disorders.
一种灵敏、非侵入性的方法来检测局部前列腺癌，特别是对于接受主动监测的患者进行早期检测和重复研究，仍然是一个未满足的需求。在这里，我们提出了一种针对前列腺特异性膜抗原（PSMA）的分子光声（PA）成像方法，PSMA 在绝大多数前列腺癌中过度表达。我们通过给予已知的 PSMA 靶向荧光剂 YC-27，在前列腺癌实验模型中进行了光谱 PA 成像，即在携带 PSMA+（PC3 PIP）和 PSMA-（PC3 flu）肿瘤的免疫功能低下的小鼠中进行。在 PA 成像时观察到 PSMA+和同基因对照肿瘤之间的对比度存在差异，PSMA+肿瘤在平均 66.07 倍的情况下显示出更高的对比度，5 只小鼠在注射后 24 小时的时间点。这些结果使用 YC-27 的标准近红外荧光成像得到了证实，PA 和荧光强度之间的平方相关性为 0.89。光谱 PA 成像是一种新的分子成像模式，具有足够的灵敏度来靶向体内的 PSMA，为其他与癌症和其他疾病相关的饱和靶标应用提供了潜力。
