Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, Brazil.
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, Brazil.
Arch Oral Biol. 2018 Jul;91:17-22. doi: 10.1016/j.archoralbio.2018.04.005. Epub 2018 Apr 6.
This study evaluated the effect of the hydroalcoholic extracts of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves (alone or combined) on the viability of Streptococcus mutans biofilm and on the prevention of enamel demineralization.
Strain of S. mutans (ATCC 21175) was reactivated in BHI broth. Minimum inhibitory concentration, minimum bactericidal concentration, minimum inhibition biofilm concentration and minimum eradication biofilm concentration were determined in order to choose the concentrations to be tested under biofilm model. S. mutans biofilm (5 × 10 CFU/ml) was produced on bovine enamel, using McBain saliva under 0.2% sucrose exposure, for 3 days. The biofilm was daily treated with the extracts for 1 min. The biofilm viability was tested by fluorescence and the enamel demineralization was measured using TMR.
Myracrodruon urundeuva All. (Isolated or combined) at the concentrationsc ≥0.625 mg/ml was able to reduce bacteria viability, while Qualea Grandflora Mart. alone had antimicrobial effect at 5 mg/ml only (p < 0.05). On the other hand, none of the extracts were able to reduce enamel demineralization.
The hydroalcoholic extracts of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves (isolated or combined) have antimicrobial action; however, they do not prevent enamel caries under S. mutans biofilm model.
本研究评估了 Myracrodruon urundeuva All. 和 Qualea grandiflora Mart. 叶的水醇提取物（单独或联合使用）对变形链球菌生物膜活力的影响，以及对预防 enamel 脱矿的作用。
将 S. mutans（ATCC 21175）菌株在 BHI 肉汤中复活。为了选择在生物膜模型下进行测试的浓度，确定了最小抑菌浓度、最小杀菌浓度、最小抑制生物膜浓度和最小消除生物膜浓度。使用 McBain 唾液在 0.2%蔗糖暴露下，在牛 enamel 上产生 5×10 CFU/ml 的 S. mutans 生物膜，持续 3 天。用提取物每天处理生物膜 1 分钟。通过荧光法测试生物膜活力，通过 TMR 测量 enamel 脱矿。
Myracrodruon urundeuva All.（单独或联合）浓度≥0.625 mg/ml 能够降低细菌活力，而 Qualea Grandflora Mart. 单独使用时仅在 5 mg/ml 时具有抗菌作用（p<0.05）。另一方面，没有一种提取物能够减少 enamel 脱矿。
Myracrodruon urundeuva All. 和 Qualea grandiflora Mart. 叶的水醇提取物（单独或联合使用）具有抗菌作用；然而，它们不能在 S. mutans 生物膜模型下预防 enamel 龋。
