杨桃和大花Qualea 叶的水醇提取物对变形链球菌生物膜和牙齿脱矿的影响。

Hydroalcoholic extracts of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves on Streptococcus mutans biofilm and tooth demineralization.

机构信息

Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, Brazil.

Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Al. Octávio Pinheiro Brisolla 9-75, Bauru, São Paulo, Brazil.

出版信息

Arch Oral Biol. 2018 Jul;91:17-22. doi: 10.1016/j.archoralbio.2018.04.005. Epub 2018 Apr 6.

DOI:10.1016/j.archoralbio.2018.04.005

PMID:29653313

Abstract

OBJECTIVES

This study evaluated the effect of the hydroalcoholic extracts of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves (alone or combined) on the viability of Streptococcus mutans biofilm and on the prevention of enamel demineralization.

METHODS

Strain of S. mutans (ATCC 21175) was reactivated in BHI broth. Minimum inhibitory concentration, minimum bactericidal concentration, minimum inhibition biofilm concentration and minimum eradication biofilm concentration were determined in order to choose the concentrations to be tested under biofilm model. S. mutans biofilm (5 × 10 CFU/ml) was produced on bovine enamel, using McBain saliva under 0.2% sucrose exposure, for 3 days. The biofilm was daily treated with the extracts for 1 min. The biofilm viability was tested by fluorescence and the enamel demineralization was measured using TMR.

RESULTS

Myracrodruon urundeuva All. (Isolated or combined) at the concentrationsc ≥0.625 mg/ml was able to reduce bacteria viability, while Qualea Grandflora Mart. alone had antimicrobial effect at 5 mg/ml only (p < 0.05). On the other hand, none of the extracts were able to reduce enamel demineralization.

CONCLUSIONS

The hydroalcoholic extracts of Myracrodruon urundeuva All. and Qualea grandiflora Mart. leaves (isolated or combined) have antimicrobial action; however, they do not prevent enamel caries under S. mutans biofilm model.

摘要

目的

本研究评估了 Myracrodruon urundeuva All. 和 Qualea grandiflora Mart. 叶的水醇提取物（单独或联合使用）对变形链球菌生物膜活力的影响，以及对预防 enamel 脱矿的作用。

方法

将 S. mutans（ATCC 21175）菌株在 BHI 肉汤中复活。为了选择在生物膜模型下进行测试的浓度，确定了最小抑菌浓度、最小杀菌浓度、最小抑制生物膜浓度和最小消除生物膜浓度。使用 McBain 唾液在 0.2%蔗糖暴露下，在牛 enamel 上产生 5×10 CFU/ml 的 S. mutans 生物膜，持续 3 天。用提取物每天处理生物膜 1 分钟。通过荧光法测试生物膜活力，通过 TMR 测量 enamel 脱矿。