Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826, Republic of Korea.
Theriogenology. 2018 Jul 1;114:191-198. doi: 10.1016/j.theriogenology.2018.03.040. Epub 2018 Apr 5.
Resveratrol and melatonin are known for their antioxidant properties and have various biological activities. The fact that they exhibit possible synergistic effects in phytomedicine researches suggests the use of a combination of these agents to promote porcine in vitro maturation (IVM) of oocytes. Therefore, we investigated the effects of resveratrol and/or melatonin on this process; cumulus-oocyte complexes underwent IVM culture with four different conditions (control, resveratrol, melatonin or their combination). Cumulus expansion, oocyte nuclear maturation and subsequent embryo development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) were evaluated. In experiment 1, all treatment groups significantly increased the proportion of complete cumulus expansion (degree 4) compared to the control, showing no difference among the treatment groups (P = 0.30). In experiment 2, oocytes matured with resveratrol and the combination had significantly higher metaphase-II (MII) rates than the control and melatonin groups, showing the highest (P < 0.05) MII rates in the combination group. In experiment 3, all treatment groups significantly increased blastocyst formation rates and total blastocyst cell numbers after PA compared to the control, but especially the combination showed the highest (P < 0.05) total cell numbers. In experiment 4, we selected the combination as the optimal condition and used this IVM system prior to SCNT. The combination treatment showed a significant (P < 0.05) increase in blastocyst formation rate and total cell numbers after SCNT. In conclusion, our results suggest that the combination of resveratrol and melatonin supported a synergistic increase in oocyte nuclear maturation and total cell numbers of PA blastocysts and improved the development of SCNT embryos.
白藜芦醇和褪黑素以其抗氧化特性而闻名，具有多种生物活性。它们在植物药研究中表现出可能的协同作用的事实表明，使用这些药物的组合来促进猪卵母细胞的体外成熟（IVM）。因此，我们研究了白藜芦醇和/或褪黑素对这一过程的影响；卵丘-卵母细胞复合物在四种不同条件下（对照、白藜芦醇、褪黑素或它们的组合）进行 IVM 培养。评估卵丘扩张、卵母细胞核成熟以及随后孤雌激活（PA）和体细胞核转移（SCNT）后的胚胎发育。在实验 1 中，与对照组相比，所有处理组的完全卵丘扩张（第 4 度）比例均显著增加，且处理组之间无差异（P=0.30）。在实验 2 中，用白藜芦醇和组合成熟的卵母细胞具有显著更高的中期-II（MII）率，高于对照组和褪黑素组，组合组显示出最高的（P<0.05）MII 率。在实验 3 中，与对照组相比，所有处理组的 PA 后囊胚形成率和总囊胚细胞数均显著增加，但组合组尤其显示出最高的（P<0.05）总细胞数。在实验 4 中，我们选择组合作为最佳条件，并在 SCNT 之前使用该 IVM 系统。组合处理显示出囊胚形成率和 SCNT 后总细胞数的显著（P<0.05）增加。总之，我们的结果表明，白藜芦醇和褪黑素的组合支持卵母细胞核成熟和 PA 囊胚总细胞数的协同增加，并改善了 SCNT 胚胎的发育。
