Kumar Rakesh, Sharma Rinku, Patil Rajendra D, Mal Gorakh, Kumar Adarsh, Patial Vikram, Kumar Pawan, Singh Bikram
Disease Investigation Laboratory, ICAR-Indian Veterinary Research Institute, Regional Station, Palampur, Himachal Pradesh, India.
DGCN COVAS, CSK HPKV, Palampur, Himachal Pradesh, India.
BMC Vet Res. 2018 Apr 13;14(1):129. doi: 10.1186/s12917-018-1444-x.
In the field conditions, animals regularly consume small quantities of lantana leaves either while grazing or due to mixing with regular fodder. The hypothesis of this study was that consumption of lantana toxins over a long period of time leads to progression of sub-clinical disease. Toxicopathological effects of sub-chronic (90 days) administration of lantadenes of L. camara were investigated in guinea pigs. For this, a total of 40 animals were divided into 5 groups whereby groups I, II, III and IV were orally administered lantadenes, daily at the dose of 24, 18, 12, and 6 mg/kg bw, respectively while group V was control. The animals were evaluated by weekly body weight changes, haematology, serum liver and kidney markers, tissue oxidative markers and histopathology.
The results of significant decrease in weekly body weights, haematology, liver and kidney marker enzymes (alanine aminotransaminase, aspartate aminotransaminase, acid phosphatase and creatinine), oxidation stress markers (lipid peroxidation, reduced glutathione, superoxide dismutase and catalase) in liver and kidneys, histopathology, and confirmation of fibrous collagenous tissue proliferation by Masson's Trichome stain showed that lantadenes led to a dose-dependent toxicity in decreasing order with the highest dose (24 mg/kg bw) producing maximum lesions and the lowest dose (6 mg/kg bw) producing minimum alterations.
The study revealed that lantadenes which are considered to be classical hepatotoxicants in acute toxicity produced pronounced nephrotoxicity during sub-chronic exposure. Further studies are needed to quantify the levels of lantadenes in blood or serum of animals exposed to lantana in field conditions which would help to assess the extent of damage to the vital organs.
在野外条件下，动物在放牧时或因与常规饲料混合而经常摄入少量马缨丹叶子。本研究的假设是，长期摄入马缨丹毒素会导致亚临床疾病的进展。在豚鼠中研究了马缨丹（L. camara）中马缨丹烯亚慢性（90天）给药的毒理病理学效应。为此，将总共40只动物分为5组，其中第I、II、III和IV组分别以24、18、12和6 mg/kg体重的剂量每日口服马缨丹烯，而第V组为对照组。通过每周体重变化、血液学、血清肝和肾标志物、组织氧化标志物和组织病理学对动物进行评估。
每周体重、血液学、肝和肾标志物酶（丙氨酸转氨酶、天冬氨酸转氨酶、酸性磷酸酶和肌酐）、肝和肾中的氧化应激标志物（脂质过氧化、还原型谷胱甘肽、超氧化物歧化酶和过氧化氢酶）显著降低，组织病理学以及通过Masson三色染色证实纤维胶原组织增生，结果表明马缨丹烯导致剂量依赖性毒性，剂量从高到低依次递减，最高剂量（24 mg/kg体重）产生最大损伤，最低剂量（6 mg/kg体重）产生最小改变。
该研究表明，在急性毒性中被认为是经典肝毒物的马缨丹烯在亚慢性暴露期间产生了明显的肾毒性。需要进一步研究来量化野外条件下接触马缨丹的动物血液或血清中马缨丹烯的水平，这将有助于评估对重要器官的损害程度。
