Disease Investigation Laboratory, Indian Veterinary Research Institute, Regional Station, Palampur 176061, Himachal Pradesh, India.
Disease Investigation Laboratory, Indian Veterinary Research Institute, Regional Station, Palampur 176061, Himachal Pradesh, India.
Toxicon. 2014 Apr;81:1-12. doi: 10.1016/j.toxicon.2014.01.013. Epub 2014 Jan 25.
The hepatoprotective effect of freeze-dried methanolic leaf extract of Ginkgo biloba was evaluated against lantadenes-induced hepatic damage in guinea pigs. The reversed-phase HPLC analysis of lantadenes confirmed the presence of 72.82% of lantadene A. UPLC-ESI-MS analysis showed the presence of ginkgolide B, C, bilobalide and traces of ginkgolide A and J in G. biloba extract. The concentration of ginkgolide B in the sample was found as 0.29%. The elevated levels of serum alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase due to lantadenes were significantly restored towards normal values by G. biloba extract in a dose-dependent manner. The effects of lantadenes and G. biloba extract on lipid peroxidation (LPO), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase were assayed in liver homogenates to evaluate the antioxidant activity. G. biloba extract in a dose-dependent manner produced significant decrease in lantadenes-induced increased levels of LPO. The lantadene-induced decreased levels of SOD, GSH and catalase were elevated by G. biloba extract. The findings of biochemical and antioxidant enzyme levels were supported by gross and histopathological observations. Moreover, liver sections of G. biloba group also showed a marked decrease in apoptosis in comparison to lantadenes group. This study suggested that G. biloba could be used as a promising hepatoprotectant against lantadenes-induced hepatic damage. Future studies are needed to elucidate the precise mechanism of hepatoprotection for practical application.
银杏叶甲醇提取物冻干物对豚鼠蓝烯诱导的肝损伤的保肝作用进行了评价。反相高效液相色谱法分析蓝烯证实了蓝烯 A 的存在比例为 72.82%。UPLC-ESI-MS 分析表明，银杏提取物中存在银杏内酯 B、C、白果内酯和痕量的银杏内酯 A 和 J。样品中银杏内酯 B 的浓度被发现为 0.29%。蓝烯导致血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶和碱性磷酸酶水平升高，银杏提取物可剂量依赖性地显著恢复至正常水平。通过测定肝匀浆中脂质过氧化 (LPO)、还原型谷胱甘肽 (GSH)、超氧化物歧化酶 (SOD) 和过氧化氢酶的变化，评估抗氧化活性。银杏提取物可剂量依赖性地降低蓝烯诱导的 LPO 水平升高。银杏提取物可升高蓝烯诱导的 SOD、GSH 和过氧化氢酶水平降低。生化和抗氧化酶水平的研究结果得到了大体和组织病理学观察的支持。此外，与蓝烯组相比，银杏组的肝组织切片显示凋亡明显减少。本研究表明，银杏可作为一种有前途的蓝烯诱导肝损伤保护剂。需要进一步的研究来阐明其确切的保肝作用机制，以便实际应用。
