利用分枝杆菌属微生物高效转化植物甾醇为去氢表雄酮
Efficient Biotransformation of Phytosterols to Dehydroepiandrosterone by Mycobacterium sp.
机构信息
College of Life Sciences, Institute of Microbial Engineering, Henan University, Kaifeng, 475004, People's Republic of China.
出版信息
Appl Biochem Biotechnol. 2018 Oct;186(2):496-506. doi: 10.1007/s12010-018-2739-x. Epub 2018 Apr 14.
In this study, a method for the efficient production of dehydroepiandrosterone (DHEA) from phytosterols in a vegetable oil/aqueous two-phase system by Mycobacterium sp. was developed. After the 3-hydroxyl group of phytosterols was protected, they could be converted into DHEA with high yield and productivity by Mycobacterium sp. NRRL B-3683. In a shake flask biotransformation, 15.05 g l of DHEA and a DHEA yield of 85.39% (mol mol) were attained after 7 days with an initial substrate concentration of 25 g l. When biotransformation was carried out in a 30-l stirred bioreactor with 25 g l substrate, the DHEA concentration and yield was 16.33 g l and 92.65% (mol mol) after 7 days, respectively. The results of this study suggest that inexpensive phytosterols could be utilized for the efficient production of DHEA.
本研究开发了一种利用分枝杆菌属（Mycobacterium sp.）从植物油/双水相体系中的植物甾醇高效生产脱氢表雄酮（DHEA）的方法。在保护植物甾醇的 3-羟基后，分枝杆菌属（Mycobacterium sp.）NRRL B-3683 可以将其转化为具有高收率和生产力的 DHEA。在摇瓶生物转化中，当初始底物浓度为 25 g/L 时，经过 7 天的反应，可获得 15.05 g/L 的 DHEA 和 85.39%（摩尔摩尔）的 DHEA 产率。当在 30 L 搅拌式生物反应器中进行生物转化时，在 7 天的反应后，DHEA 的浓度和产率分别为 16.33 g/L 和 92.65%（摩尔摩尔）。本研究结果表明，廉价的植物甾醇可用于高效生产 DHEA。
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