Laboratoire de Chimie Organique et Bioorganique, Institut de Recherche Jean-Baptiste Donnet, Université de Haute Alsace, EA4566, 3 rue Alfred Werner, 68093, Mulhouse, France.
J Ind Microbiol Biotechnol. 2013 Nov;40(11):1315-20. doi: 10.1007/s10295-013-1315-1. Epub 2013 Aug 18.
Bioconversion of lipophilic compounds poorly soluble in water, such as sterols, required the use of chemicals and solubilizing agents. On the other hand, it was shown that immobilization of Mycobacterium species on the dried fruit of Luffa cylindrica (DFLC) allows a close interaction between immobilized cells and cholesterol particles and increases by then the product's yield. In this work, the use of DFLC in a 5-l jar bioreactor with phytosterols mixture (1 g/l) as substrate was assessed without addition of any chemicals or solubilizing agents. DFLC increased by a factor of four the volumetric productivity of androstenones (0.08 g/l day). Products were accumulated in the aqueous medium while substrates remained on the fibers of DFLC. This observation lets envisage a green semi-continuous process of androstenone production. DFLC has no influence on cell growth, and is moreover natural, inexpensive, non-toxic, and mechanically strong.
疏水性化合物（如固醇）在水中的溶解度较差，因此需要使用化学物质和增溶剂进行生物转化。另一方面，已经证明将分枝杆菌属固定在丝瓜络（DFLC）的干果实上可以使固定化细胞与胆固醇颗粒密切相互作用，并提高产品的产量。在这项工作中，在没有添加任何化学物质或增溶剂的情况下，使用 5 升罐生物反应器中的 DFLC 来评估植物固醇混合物（1 g/l）作为底物。DFLC 将雄甾酮的比体积生产率提高了四倍（0.08 g/l 天）。产物在水相介质中积累，而底物则留在 DFLC 的纤维上。这一观察结果使得可以设想一种绿色的半连续雄甾酮生产工艺。DFLC 对细胞生长没有影响，而且它天然、廉价、无毒且机械强度高。
