School of Medicine, Department of Medical Biochemistry, Koc University, Rumelifeneri Yolu, Sariyer, 34450, Istanbul, Turkey.
Faculty of Science, Department of Biology, Dumlupınar University, Kütahya, Turkey.
Biol Trace Elem Res. 2018 Dec;186(2):474-488. doi: 10.1007/s12011-018-1331-0. Epub 2018 Apr 13.
Di(2-ethylhexyl) phthalate (DEHP) is a widely used synthetic polymer in the industry. DEHP may induce reproductive and developmental toxicity, obesity, carcinogenesis and cause abnormal endocrine function in both human and wildlife. The aim of this study was to investigate trace element and mineral levels in relation of kidney and liver damage in DEHP-administered rats. Therefore, prepubertal male rats were dosed with 0, 100, 200, and 400 mg/kg/day of DEHP. At the end of the experiment, trace element and mineral levels, glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6-PGD), glutathione reductase (GR) and glutathione S-transferase (GST) enzyme activities were evaluated in the serum, liver, and kidney samples of rats. Furthermore, serum clinical biochemistry parameters, organ/body weight ratios and histological changes were investigated to evaluate impact of DEHP more detailed. Our data indicated that sodium (Na), calcium (Ca), potassium (K), lithium (Li), rubidium (Rb) and cesium (Cs) levels significantly decreased, however iron (Fe) and selenium (Se) concentrations significantly increased in DEHP-administered groups compared to the control in the serum samples. On the other hand, upon DEHP administration, selenium concentration, G6PD and GR activities were significantly elevated, however 6-PGD activity significantly decreased compared to the control group in the kidney samples. Decreased G6PD activity was the only significant change between anti-oxidant enzyme activities in the liver samples. Upon DEHP administration, aberrant serum biochemical parameters have arisen and abnormal histological changes were observed in the kidney and liver tissue. In conclusion, DEHP may induce liver and kidney damage, also result abnormalities in the trace element and mineral levels.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是工业中广泛使用的合成聚合物。DEHP 可能会引起生殖和发育毒性、肥胖、致癌作用，并导致人类和野生动物的内分泌功能异常。本研究旨在探讨与 DEHP 给药大鼠的肝肾损伤相关的微量元素和矿物质水平。因此，青春期前雄性大鼠分别给予 0、100、200 和 400mg/kg/天的 DEHP。实验结束时，评估了大鼠血清、肝脏和肾脏样本中的微量元素和矿物质水平、葡萄糖-6-磷酸脱氢酶（G6PD）、6-磷酸葡萄糖酸脱氢酶（6-PGD）、谷胱甘肽还原酶（GR）和谷胱甘肽 S-转移酶（GST）酶活性。此外，还研究了血清临床生化参数、器官/体重比和组织学变化，以更详细地评估 DEHP 的影响。我们的数据表明，与对照组相比，血清样本中钠（Na）、钙（Ca）、钾（K）、锂（Li）、铷（Rb）和铯（Cs）的水平显著降低，而铁（Fe）和硒（Se）的浓度显著增加。另一方面，与对照组相比，给予 DEHP 后，肾脏样本中的硒浓度、G6PD 和 GR 活性显著升高，而 6-PGD 活性显著降低。肝脏样本中抗氧化酶活性只有 G6PD 活性的变化具有统计学意义。给予 DEHP 后，血清生化参数异常，肾脏和肝脏组织出现异常组织学变化。总之，DEHP 可能会引起肝肾功能损害，还会导致微量元素和矿物质水平异常。
