用于食品代谢组学应用的核磁共振光谱分析方案

NMR Spectroscopy Protocols for Food Metabolomics Applications.

作者信息

Ralli Evangelia, Amargianitaki Maria, Manolopoulou Efi, Misiak Maria, Markakis Georgios, Tachtalidou Sofia, Kolesnikova Alexandra, Dais Photis, Spyros Apostolos

机构信息

NMR Laboratory, Chemistry Department, University of Crete, Heraklion, Crete, Greece.

出版信息

Methods Mol Biol. 2018;1738:203-211. doi: 10.1007/978-1-4939-7643-0_14.

DOI:10.1007/978-1-4939-7643-0_14

PMID:29654592

Abstract

NMR spectroscopy has become an indispensable tool for the metabolic profiling of foods and food products. In the present protocol, we report an analytical approach based on liquid-state NMR for the determination of polar and nonpolar metabolites in some common liquid (wine, spirits, juice) and solid (cheese, coffee, honey) foods. Although the diversity of foods precludes the use of a single protocol, with small modifications, the proposed methodologies can be adapted to a broader range of foodstuffs.

摘要

核磁共振光谱已成为食品和食品产品代谢物分析的不可或缺的工具。在本方案中，我们报告了一种基于液态核磁共振的分析方法，用于测定一些常见液体（葡萄酒、烈酒、果汁）和固体（奶酪、咖啡、蜂蜜）食品中的极性和非极性代谢物。尽管食品种类繁多，无法使用单一方案，但只需稍作修改，所提出的方法就可适用于更广泛的食品。