Morris L H A
EquiBreed NZ Ltd, Te Awamutu, New Zealand.
Equine Vet J. 2018 Nov;50(6):712-720. doi: 10.1111/evj.12839. Epub 2018 May 25.
The development of techniques to produce equine embryos in vitro is reviewed with specific reference to intracytoplasmic sperm injection (ICSI). Unexplored 50 years ago, this technology has progressed rapidly in the last 20 years to become a commercial reality for the equine breeding industry. Improvements in our understanding of oocyte and embryo competence in the horse have been key factors in overcoming some of the initial problems associated with ICSI. It is now possible to obtain high nuclear maturation and cleavage rates in vitro and the most limiting factor, presently, is the low rate of development to the blastocyst stage. However, in spite of this, once obtained, these in vitro-derived blastocysts can result in pregnancy rates in excess of 60% following transfer.
本文回顾了马胚胎体外生产技术的发展，特别提及了胞浆内单精子注射（ICSI）技术。这项技术在50年前还未被探索，在过去20年里却迅速发展，已成为马繁育行业的商业现实。我们对马的卵母细胞和胚胎发育能力的认识有所提高，这是克服与ICSI相关的一些初始问题的关键因素。现在，体外已能够获得较高的核成熟率和卵裂率，目前最限制因素是发育至囊胚阶段的比率较低。然而，尽管如此，一旦获得这些体外来源的囊胚，移植后的妊娠率可超过60%。
