Li Y, Mou Y, Thunders M, Wu Y, Ai X, Zhou X, Qiu J
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
College of Health, Massey University, Wellington, New Zealand.
J Vet Pharmacol Ther. 2018 Aug;41(4):562-571. doi: 10.1111/jvp.12493. Epub 2018 Apr 14.
Enrofloxacin (EF) is a widely used fluoroquinolone, usually regarded as a safe and effective treatment for bacterial infections. Adverse effects of EF have previously been demonstrated in some species, but so far there have been no studies looking specifically at the impact of EF on pigs. In this study, three different doses of EF (5, 25 and 125 mg kg bw ) were administrated to Bama pigs. The results showed that lipid peroxidation of pig liver tissue occurred with all EF doses. The 125 mg kg dose of EF induced catalase (CAT) and glutathione peroxidase (GSH-px) and increased CYP450 content in pig liver microsomes. The activity of microsomal NADPH-cytochrome C reductase (NCCR) was elevated at both the 25 and 125 mg kg doses of EF. Microsomal erythromycin N-demethylase (ERND) and aminopyrin N-demethylase (AND) were inhibited by high doses of EF, while aniline-4-hydroxylase (AH) was unaffected. None of the EF treatments affected superoxide dismutase (SOD) or cytochrome b5 content. Antioxidases and microsomal enzymes may work together to resist the adverse effects of EF. Proteomic analysis revealed increased protein expression of carboxylesterase (CES) and alpha-enolase (ENO1) in microsomes as a stress response to EF. These results provide new information about the adverse effect of fluoroquinolones and help guide their usage more effectively in the clinic or animal breeding.
恩诺沙星（EF）是一种广泛使用的氟喹诺酮类药物，通常被认为是治疗细菌感染的安全有效药物。EF的不良反应此前已在一些物种中得到证实，但迄今为止，尚未有专门研究EF对猪影响的报道。在本研究中，对巴马猪施用了三种不同剂量的EF（5、25和125毫克/千克体重）。结果表明，所有EF剂量均导致猪肝组织发生脂质过氧化。125毫克/千克剂量的EF诱导了过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-px），并增加了猪肝微粒体中的细胞色素P450含量。在25和125毫克/千克剂量的EF作用下，微粒体NADPH-细胞色素C还原酶（NCCR）的活性均升高。高剂量的EF抑制了微粒体红霉素N-脱甲基酶（ERND）和氨基比林N-脱甲基酶（AND），而苯胺-4-羟化酶（AH）未受影响。所有EF处理均未影响超氧化物歧化酶（SOD）或细胞色素b5的含量。抗氧化酶和微粒体酶可能共同作用以抵抗EF的不良反应。蛋白质组学分析显示，微粒体中羧酸酯酶（CES）和α-烯醇化酶（ENO1）的蛋白质表达增加，作为对EF的应激反应。这些结果提供了有关氟喹诺酮类药物不良反应的新信息，并有助于在临床或动物养殖中更有效地指导其使用。
