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二氧化氯对猪肠上皮细胞基因标志物的体外研究。

In vitro study of chlorine dioxide on porcine intestinal epithelial cell gene markers.

机构信息

Department of Pharmacology and Toxicology, University of Veterinary Medicine, Budapest, Hungary.

Department of Physics, Faculty of Natural Sciences, Budapest University of Technology and Economics, Budapest, Hungary.

出版信息

Vet Med Sci. 2022 Mar;8(2):591-597. doi: 10.1002/vms3.658. Epub 2021 Oct 20.

DOI:10.1002/vms3.658
PMID:34672097
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8959260/
Abstract

BACKGROUND

Chlorine dioxide (ClO ) is an inorganic, potent biocide and is available in highly purified aqueous solution. It can be administered as an oral antiseptic in this form.

OBJECTIVES

Our aim is to determine the level of inflammatory markers and cytochrome genes expressed by enterocytes exposed to different concentrations of hyperpure chlorine dioxide solution.

METHODS

Porcine jejunal enterocyte cell (IPEC-J2) cultures were treated with the aqueous solution of hyper-pure chlorine dioxide of various concentrations. We determined the alterations in mRNA levels of inflammatory mediators, such as IL6, CXCL8/IL8, TNF, HSPA6 (Hsp70), CAT and PTGS2 (COX2); furthermore, the expression of three cytochrome genes (CYP1A1, CYP1A2, CYP3A29) were analysed by quantitative PCR method.

RESULTS

The highest applied ClO concentration reduced the expression of all three investigated CYP genes. The gene expression of PTGS2 and CAT were not altered by most concentrations of ClO . The expression of IL8 gene was reduced by all applied concentrations of ClO . TNF mRNA level was also decreased by most ClO concentrations used.

CONCLUSIONS

Different concentrations of chlorine dioxide exhibited immunomodulatory activity and caused altered transcription of CYP450 genes in porcine enterocytes. Further studies are needed to determine the appropriate ClO concentration for oral use in animals.

摘要

背景

二氧化氯（ClO ）是一种无机的强效杀菌剂，以高纯度水溶液形式存在。它可以以这种形式作为口服防腐剂使用。

目的

我们旨在确定暴露于不同浓度超纯二氧化氯溶液的肠细胞表达的炎症标志物和细胞色素基因的水平。

方法

用不同浓度的超纯二氧化氯水溶液处理猪空肠肠细胞（IPEC-J2）培养物。我们测定了炎性介质（如 IL6、CXCL8/IL8、TNF、HSPA6（Hsp70）、CAT 和 PTGS2（COX2））的 mRNA 水平的变化；此外，还通过定量 PCR 法分析了三种细胞色素基因（CYP1A1、CYP1A2、CYP3A29）的表达。

结果

应用的最高 ClO 浓度降低了所有三种研究的 CYP 基因的表达。PTGS2 和 CAT 的基因表达不受大多数 ClO 浓度的影响。IL8 基因的表达被所有应用的 ClO 浓度降低。TNF mRNA 水平也被大多数使用的 ClO 浓度降低。

结论

不同浓度的二氧化氯表现出免疫调节活性，并导致猪肠细胞中 CYP450 基因的转录发生改变。需要进一步的研究来确定动物口服使用的适当 ClO 浓度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eaa/8959260/70da3b1ebf64/VMS3-8-591-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eaa/8959260/af49f55faf5e/VMS3-8-591-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eaa/8959260/134b837586a9/VMS3-8-591-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eaa/8959260/549148199b95/VMS3-8-591-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eaa/8959260/d4b58e5bf215/VMS3-8-591-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eaa/8959260/70da3b1ebf64/VMS3-8-591-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eaa/8959260/af49f55faf5e/VMS3-8-591-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eaa/8959260/134b837586a9/VMS3-8-591-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eaa/8959260/549148199b95/VMS3-8-591-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eaa/8959260/d4b58e5bf215/VMS3-8-591-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6eaa/8959260/70da3b1ebf64/VMS3-8-591-g001.jpg

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本文引用的文献

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Mediators Inflamm. 2019 Jan 2;2019:5420381. doi: 10.1155/2019/5420381. eCollection 2019.
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Hsp70 Suppresses Mitochondrial Reactive Oxygen Species and Preserves Pulmonary Microvascular Barrier Integrity Following Exposure to Bacterial Toxins.
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