Li Kun, Shahzad Muhammad, Zhang Hui, Mehmood Khalid, Jiang Xiong, Luo Houqiang, Zhang Lihong, Dong Xiaoqian, Li Jiakui
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, People's Republic of China.
University College of Veterinary & Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur, Pakistan.
Acta Parasitol. 2018 Jun 26;63(2):280-286. doi: 10.1515/ap-2018-0032.
The present study was designed to determine and analyze the mt genomes of Metastrongylus salmi (M. salmi), and reveal the phylogenetic relationships of this parasite using mt DNA sequences. Results showed that the complete mt genome of M. salmi was 13722 bp containing 12 protein-coding genes (cox1-3, nad1-6, nad4L, atp6 and cytb), 22 transfer RNA genes, and 2 ribosomal RNA genes (rrnL and rrnS). The overall A+T content was 73.54% and the nucleotide composition was A (23.52%), C (6.14%), G (19.60%), T (50.02%), and N (UCAG) (0.73%). A total of 4237 amino acids are encoded from the Tibetan isolates of M. salmi mt genomes. The ATA was predicted as the most common starting codon with 41.7% (5/12 protein genes); and 11 of the 12 protein genes were found to have a TAG or TAA translation termination codon. By clustering together the phylogenetic trees of Tibetan M. salmi and Austrian M. salmi, the M. salmi isolated from Tibetan pigs was found to be highly homological with that stemmed from Austrian one. This information provides meaningful insights into the phylogenetic position of the M. salmi China isolate and represents a useful resource for selecting molecular markers for diagnosis and population studies.
本研究旨在测定和分析萨尔米后圆线虫（M. salmi）的线粒体基因组，并利用线粒体DNA序列揭示该寄生虫的系统发育关系。结果表明，萨尔米后圆线虫的完整线粒体基因组为13722 bp，包含12个蛋白质编码基因（cox1 - 3、nad1 - 6、nad4L、atp6和cytb）、22个转运RNA基因和2个核糖体RNA基因（rrnL和rrnS）。总体A + T含量为73.54%，核苷酸组成为A（23.52%）、C（6.14%）、G（19.60%）、T（50.02%）和N（UCAG）（0.73%）。萨尔米后圆线虫线粒体基因组的西藏分离株共编码4237个氨基酸。ATA被预测为最常见的起始密码子，占41.7%（12个蛋白质基因中的5个）；12个蛋白质基因中有11个具有TAG或TAA翻译终止密码子。通过将西藏萨尔米后圆线虫和奥地利萨尔米后圆线虫的系统发育树聚类在一起，发现从西藏猪分离的萨尔米后圆线虫与源自奥地利的萨尔米后圆线虫高度同源。该信息为中国分离的萨尔米后圆线虫的系统发育位置提供了有意义的见解，并为选择用于诊断和种群研究的分子标记提供了有用的资源。
