Laboratory of Epidemiology and Molecular Systematics, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
Oswaldo Cruz Foundation Regional Office-Piauí, Teresina, Piauí, Brazil.
J Vet Diagn Invest. 2022 Jul;34(4):689-692. doi: 10.1177/10406387221092143. Epub 2022 May 24.
Metastrongylosis is an infection of the respiratory tract of pigs caused by parasites of the genus , whose eggs are similar to other through light microscopy; species-specific identification can be performed with molecular tools. We explored the species composition and the genetic diversity of infecting pigs in close contact with humans in impoverished rural communities in the state of Piauí, in northeastern Brazil. Fecal samples ( = 78) were collected for parasitologic tests. Egg morphometry and molecular characterization, using the cytochrome c oxidase subunit 1 (1) gene, were performed. For strongyliform eggs, 62 of 78 (80%) pigs were positive and 6 of 99 (6%) eggs had dimensions compatible with . Of the 37 samples submitted to PCR, 10 were identified as . We found 3 haplotypes, including 2 new and 1 described previously in Europe. Overall, demonstrated lower intraspecific genetic diversity: diversity index (H) ± SD = 0.318 ± 0.164, = 12, compared with published sequences (1.000 ± 0.272, = 3). To our knowledge, DNA sequences have not been published previously from pigs in South America.
类圆线虫病是一种由类圆线虫属寄生虫引起的猪呼吸道感染，其卵类似于其他类圆线虫通过光镜观察；种特异性鉴定可以使用分子工具进行。我们探索了与人类密切接触的贫困农村社区中感染猪的种属组成和遗传多样性，这些社区位于巴西东北部皮奥伊州。收集了 78 份粪便样本进行寄生虫学检测。进行了卵形态测量和分子特征分析，使用细胞色素 c 氧化酶亚基 1（1）基因。对于类圆线虫卵，78 份样本中有 62 份（80%）为阳性，99 份卵中有 6 份（6%）的尺寸与相符。在提交进行 PCR 的 37 个样本中，有 10 个被鉴定为。我们发现了 3 个单倍型，包括 2 个新的和 1 个之前在欧洲描述过的。总体而言，显示出较低的种内遗传多样性：多样性指数（H）±标准差（SD）=0.318±0.164，N=12，与已发表的序列相比（1.000±0.272，N=3）。据我们所知，以前从未从南美的猪中公布过的 DNA 序列。
