College of Agriculture and Forestry Science, Linyi University, Linyi 276005, China.
College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Biomed Res Int. 2020 Jan 13;2020:7975948. doi: 10.1155/2020/7975948. eCollection 2020.
A study was conducted to reveal the characterization of the complete mitochondrial genome of derived from cows in Shanghai, China. Results indicated that the complete genome of was 14,288 bp and contained 12 protein-coding genes (, , , , and ), 22 transfer RNA genes, and two ribosomal RNA genes (l-rRNA and s-rRNA). The overall A + T content of the genome was 63.83%, and the nucleotide composition was A (19.83%), C (9.75%), G (26.43%), and T (44.00%). A total of 3284 amino acids were encoded by current isolate genome, TTT (Phe) (9.84%) and TTG (Leu) (7.73%) codon were the most frequent amino acids, whereas the ACC (Thr) (0.06%), GCC (Ala) (0.09%), CTC (Leu) (0.09%), and AAC (Asn) (0.09%) codon were the least frequent ones. At the third codon position of protein genes, T (50.82%) was observed most frequently and C (5.85%) was the least one. The current results can contribute to epidemiology diagnosis, molecular identification, taxonomy, genetic, and drug development researches about this parasite species in cattle.
一项研究旨在揭示源自中国上海奶牛的 的完整线粒体基因组特征。结果表明， 的完整 基因组为 14288bp，包含 12 个蛋白编码基因（ 、 、 、 、和 ）、22 个转移 RNA 基因和两个核糖体 RNA 基因（l-rRNA 和 s-rRNA）。 的基因组 A+T 含量总体为 63.83%，核苷酸组成分别为 A（19.83%）、C（9.75%）、G（26.43%）和 T（44.00%）。当前 分离株基因组共编码 3284 个氨基酸，TTT（Phe）（9.84%）和 TTG（Leu）（7.73%）密码子是最常见的氨基酸，而 ACC（Thr）（0.06%）、GCC（Ala）（0.09%）、CTC（Leu）（0.09%）和 AAC（Asn）（0.09%）密码子是最少见的。在 蛋白基因的第三密码子位置，观察到 T（50.82%）最频繁，而 C（5.85%）最不频繁。目前的研究结果可为该寄生虫在牛中的流行病学诊断、分子鉴定、分类学、遗传学和药物开发研究提供参考。
