Barbosa Alynne da Silva, Barbosa Helene Santos, Souza Sandra Maria de Oliveira, Dib Laís Verdan, Uchôa Claudia Maria Antunes, Bastos Otilio Machado Pereira, Amendoeira Maria Regina Reis
Laboratório de Toxoplasmose e outras Protozooses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, Brasil, CEP: 21045900.
Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia. Rua Professor Hernani de Mello, São Domingos, Niterói, Rio de Janeiro, Brasil, CEP: 24210130.
Acta Parasitol. 2018 Jun 26;63(2):287-298. doi: 10.1515/ap-2018-0033.
Balantioides coli is a ciliated protozoon that inhabits the intestine of pigs, non-human primates and humans. Light microscopy studies have described over 50 species of the genus Balantioides but their validity is in doubt. Due to the limited information about this genus, this study is aimed to identify morphological characteristics of Balantioides coli isolated using fluorescence microscopy and both scanning (SEM) and transmission electron microscopy (TEM). Trophozoites isolated from the feces of pig and macaque were washed and subjected to centrifugation. These cells were fixed with paraformaldehyde for immunofluorescence. Other aliquots of these trophozoites were fixed with glutaraldehyde, post fixed with osmium tetroxide and processed for SEM and TEM. Immunofluorescence studies revealed microtubules with a longitudinal distribution to the main axis of the parasite and in the constitution of cilia. SEM demonstrated a high concentration of cilia covering the oral apparatus and a poor presence of such structures in cytopyge. TEM revealed in the plasma membrane, several associated structures were observed to delineate the cellular cortex and mucocysts. The cytoskeleton of the oral region was observed in detail and had an organization pattern consisting of microtubules, which formed files and nematodesmal networks. Organelles such as hydrogenosomes like and peroxisomes were observed close to the cortex. Macronuclei were observed, but structures that were consistent with micronuclei were not identified. Ultrastructural morphological analysis of isolates confirms its similarity to Balantioides coli. In this study were identified structures that had not yet been described, such as hydrogenosomes like and cytoskeletal structures.
结肠小袋纤毛虫是一种纤毛原生动物，寄生于猪、非人灵长类动物和人类的肠道。光学显微镜研究描述了50多种结肠小袋纤毛虫属物种，但其有效性存疑。由于关于该属的信息有限，本研究旨在通过荧光显微镜以及扫描电子显微镜（SEM）和透射电子显微镜（TEM）来鉴定分离出的结肠小袋纤毛虫的形态特征。从猪和猕猴粪便中分离出的滋养体经过洗涤和离心处理。这些细胞用多聚甲醛固定用于免疫荧光。这些滋养体的其他等分试样用戊二醛固定，再用四氧化锇后固定，然后进行扫描电子显微镜和透射电子显微镜处理。免疫荧光研究显示，微管沿寄生虫主轴纵向分布且存在于纤毛结构中。扫描电子显微镜显示，口器上覆盖着大量纤毛，而胞肛处此类结构较少。透射电子显微镜显示，在质膜中观察到几种相关结构，它们勾勒出细胞皮层和黏液囊。详细观察了口区的细胞骨架，其组织模式由微管组成，形成了纵列和丝状网络。在靠近皮层处观察到了类氢化酶体和过氧化物酶体等细胞器。观察到了大核，但未发现与小核一致的结构。分离株的超微结构形态分析证实其与结肠小袋纤毛虫相似。在本研究中鉴定出了尚未描述过的结构，如类氢化酶体和细胞骨架结构。
