外泌体介导的真皮乳头细胞调控毛囊发育。
Regulation of hair follicle development by exosomes derived from dermal papilla cells.
机构信息
Department of Dermatology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
Department of Dermatology, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
出版信息
Biochem Biophys Res Commun. 2018 Jun 2;500(2):325-332. doi: 10.1016/j.bbrc.2018.04.067. Epub 2018 Apr 13.
BACKGROUND
Dermal papilla cells (DPCs) play a critical role in the regulation of hair follicle (HF) growth, formation, and cycling. DPCs are thought to regulate HF growth through a paracrine mechanism, in which exosomes may play a critical role.
METHODS
DPC-Exos were cutaneously injected into HFs at different HF cycle stages and the effects were evaluated by histological and immunohistochemical analyses. The effects of DPC-Exos on proliferation, migration, and cell cycle status of outer root sheath cells (ORSCs) were evaluated. After treatment of DPC-Exos, changes in mRNA and protein levels of β-catenin and Sonic hedgehog (Shh) in ORSCs were detected.
RESULTS
DPC-Exos were approximately 105 nm in diameter and expressed tumor susceptibility gene 101, cluster of differentiation (CD)9, and CD63. Injection of DPC-Exos accelerated the onset of HF anagen and delayed catagen in mice. Immunohistochemical analyses revealed that β-catenin and Shh levels were upregulated in the skin. In vitro, DPC-Exo treatment enhanced ORSC proliferation and migration, and stimulated the expression of β-catenin and Shh.
CONCLUSION
DPC-Exos contribute to the regulation of HF growth and development, and provide a potential avenue for the treatment of hair loss.
背景
真皮乳头细胞（DPC）在毛囊（HF）生长、形成和周期调控中起着关键作用。DPC 被认为通过旁分泌机制调节 HF 生长，其中外泌体可能起着关键作用。
方法
将 DPC-Exos 注射到不同 HF 周期阶段的 HF 中，并通过组织学和免疫组织化学分析评估其效果。评估 DPC-Exos 对毛鞘细胞（ORSCs）增殖、迁移和细胞周期状态的影响。在处理 DPC-Exos 后，检测 ORSCs 中β-连环蛋白和 Sonic hedgehog（Shh）的 mRNA 和蛋白水平变化。
结果
DPC-Exos 的直径约为 105nm，表达肿瘤易感性基因 101、分化（CD）9 和 CD63。DPC-Exos 的注射加速了小鼠 HF 生长期的开始，并延迟了 HF 退行期的开始。免疫组织化学分析显示皮肤中β-连环蛋白和 Shh 水平上调。在体外，DPC-Exo 处理增强了 ORSC 的增殖和迁移，并刺激了β-连环蛋白和 Shh 的表达。
结论
DPC-Exos 有助于调节 HF 的生长和发育，为治疗脱发提供了一种潜在的途径。
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